Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DELTAD-arm | tRNAPhe variant from yeast with a modification in the D-stem, inhibition | Thermus thermophilus | |
G18A | tRNAPhe variant from yeast with an exchange of G to A at position 18, inhibition | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview: Km of 28 tRNAPhe variants from yeast | Thermus thermophilus | |
0.000005 | - |
guanosine18 in Escherichia coli tRNA-Met | - |
Thermus thermophilus | |
0.000005 | - |
guanosine18 in yeast tRNA-Phe | below | Thermus thermophilus | |
0.000006 | - |
Escherichia coli tRNA-Ile | - |
Thermus thermophilus | |
0.00001 | - |
guanosine18 in Escherichia coli tRNA-Phe | - |
Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | substrate recognition sites and mechanism | Thermus thermophilus | |
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | G18, G19 and the D-stem of the tRNA are required for Gm-methylase recognition | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus | ? | - |
? | |
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
S-adenosyl-L-methionine + Escherichia coli tRNA-Met | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Met | - |
? | |
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Ile | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Ile | - |
? | |
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | substrate tRNAs from E. coli, yeast, Bacillus subtilis | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | substrate tRNAs from E. coli, yeast, Bacillus subtilis | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00079 | - |
G18A | - |
Thermus thermophilus | |
0.00205 | - |
DELTAD-arm | - |
Thermus thermophilus |