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Literature summary for 2.1.1.34 extracted from

  • Hori, H.; Matsumoto, T.; Watanabe, K.; Ohta, T.; Miura, K.
    Two types of tRNA(Gm)methylase found in extreme thermophile, Thermus thermophilus strains HB 8 and HB 27 (1985), Nucleic Acids Symp. Ser., 16, 225-228.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
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Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
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additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
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?
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
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?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
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yeast tRNAPhe Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 strain HB 8 shows 80% activity at 40°C, strain HB 27 shows 60% activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
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Thermus thermophilus strain HB27: 40% of maximal activity, strain HB8: 80% of maximal activity Thermus thermophilus