Information on EC 1.3.1.42 - 12-oxophytodienoate reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.3.1.42
-
RECOMMENDED NAME
GeneOntology No.
12-oxophytodienoate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alpha-Linolenic acid metabolism
-
-
Biosynthesis of secondary metabolites
-
-
jasmonic acid biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate:NADP+ 4-oxidoreductase
Involved in the conversion of linolenate into jasmonate in Zea mays.
CAS REGISTRY NUMBER
COMMENTARY hide
101150-03-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
oat, L. cv Moore
-
-
Manually annotated by BRENDA team
L. cv Sundak
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
flax, L. cv Linott
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
egg plant
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
wheat, L. cv Thatcher
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
(2E)-3,7-dimethylocta-2,6-dienal + NADH
(3S)-3,7-dimethyloct-6-enal + NAD+
show the reaction diagram
(2E)-3,7-dimethylocta-2,6-dienal + NADPH
(3S)-3,7-dimethyloct-6-enal + NADP+
show the reaction diagram
(2Z)-2-methylbut-2-enedioic acid + NADH
(2R)-2-methylbutanedioic acid + NAD+
show the reaction diagram
above 99% conversion with OPR-1
-
-
?
(2Z)-2-methylbut-2-enedioic acid + NADPH
(2R)-2-methylbutanedioic acid + NADP+
show the reaction diagram
above 99% conversion with OPR-1
-
-
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
-
enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid
-
-
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
?
show the reaction diagram
-
FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
-
-
?
(9S,13S)-12-oxophytodienoate + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
-
-
-
?
(9S,13S)-12-oxophytodienoic acid + NADPH + H+
3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
OPR3 is involved in jasmonic acid biosynthesis
-
-
?
(E)-2-hexenal + NADPH + H+
hexanal + NADP+
show the reaction diagram
-
-
-
-
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
12-oxo-phytodienoic acid + NADPH
3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+
show the reaction diagram
enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway
-
-
?
12-oxo-trans-phythodienoic acid + alpha-NADPH
trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
show the reaction diagram
-
-
-
-
?
12-oxo-trans-phythodienoic acid + beta-NADPH
trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
show the reaction diagram
-
-
-
-
?
12-oxo-trans-phythodienoic acid + NADPH
t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
show the reaction diagram
15-deoxy-DELTA12,14-prostaglandin J2 + alpha-NADPH
? + alpha-NADPH
show the reaction diagram
-
PGJ2, an analogue of OPDA, rates of reaction with alpha-NADPH twenty times lower than those with beta-NADPH
-
-
?
15-deoxy-DELTA12,14-prostaglandin J2 + beta-NADPH
? + NADP+
show the reaction diagram
-
PGJ2, an analogue of OPDA
-
-
?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+
show the reaction diagram
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+
show the reaction diagram
2-cyclohexen-1-ol + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
2-cyclohexen-1-one + NADH
? + NAD+
show the reaction diagram
-
-
-
-
?
2-cyclohexen-1-one + NADPH
? + NADP+
show the reaction diagram
2-methylcyclopent-2-en-1-one + NADH
(2S)-2-methylcyclopentanone + NAD+
show the reaction diagram
2-methylcyclopent-2-en-1-one + NADPH
(2S)-2-methylcyclopentanone + NADP+
show the reaction diagram
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
show the reaction diagram
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
show the reaction diagram
3-methylcyclopent-2-en-1-one + NADH
(3S)-3-methylcyclopentanone + NAD+
show the reaction diagram
2% conversion with OPR-3
-
-
?
3-methylcyclopent-2-en-1-one + NADPH
(3S)-3-methylcyclopentanone + NADP+
show the reaction diagram
1% conversion with OPR-3
-
-
?
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
?
show the reaction diagram
-
in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
-
-
-
9R,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
show the reaction diagram
-
oxylipin
-
-
?
9S,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
show the reaction diagram
-
does not utilize 13S-configured isomers
-
-
?
beta-oestradiol + NADPH
? + NADP+
show the reaction diagram
codeinone + NADH
hydrocodone + NAD+
show the reaction diagram
-
-
-
-
?
cyclohexenone + alpha-NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
cyclohexenone + beta-NADPH
?
show the reaction diagram
maleic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
morphinone + NADH
hydromorphone + NAD+
show the reaction diagram
-
-
-
-
?
oxylipin + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
racemic 12-oxo-phytodienoic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
testosterone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
trans-hex-2-enal + NADPH
hexanal + NADP+
show the reaction diagram
-
-
-
?
[(E)-1-methyl-2-nitroethenyl]benzene + NADH
[(1R)-1-methyl-2-nitroethyl]benzene + NAD+
show the reaction diagram
above 99% conversion with OPR-1
-
-
?
[(E)-1-methyl-2-nitroethenyl]benzene + NADH
[(1S)-1-methyl-2-nitroethyl]benzene + NAD+
show the reaction diagram
69% conversion with OPR-3
-
-
?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH
[(1R)-1-methyl-2-nitroethyl]benzene + NADP+
show the reaction diagram
above 99% conversion with OPR-1
-
-
?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH
[(1S)-1-methyl-2-nitroethyl]benzene + NADP+
show the reaction diagram
72% conversion with OPR-3
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
Q9FEW9, Q9FEX0, Q9XG54
-
-
-
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
show the reaction diagram
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
-
enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid
-
-
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
?
show the reaction diagram
-
FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
-
-
?
(9S,13S)-12-oxophytodienoic acid + NADPH + H+
3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
Q8GYB8, Q8LAH7, Q9FUP0
OPR3 is involved in jasmonic acid biosynthesis
-
-
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
show the reaction diagram
12-oxo-phytodienoic acid + NADPH
3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+
show the reaction diagram
Q84QK0
enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway
-
-
?
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
?
show the reaction diagram
-
in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
-
-
-
9R,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
show the reaction diagram
-
oxylipin
-
-
?
9S,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
show the reaction diagram
-
does not utilize 13S-configured isomers
-
-
?
oxylipin + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
NADPH
additional information
in the dimer interface of enzyme, a sulfate ion is bound which forms hydrogen bonds with two arginines of protomer A and one arginine of loop L6 of the partner protomer B. Sulfate ion may mimic the phosphate group of phosphorylated T364
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cortisone
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p-Hydroxybenzaldehyde
-
;
progesterone
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-trinitrotoluene
expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 17fold up-regulation of OPR1 in the root; expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 6fold up-regulation of OPR2 in the root
cis-3-hexen-1-yl acetate
OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.043
(15Z)-12-oxophyto-10,15-dienoate
pH 7.5, 25°C, recombinant enzyme
0.035
(9S,13S)-12-oxophytodienoic acid
-
-
0.045
12-oxo-cis-phythodienoic acid
-
-
0.19
12-oxophytodienoic acid
-
-
0.05283 - 0.148
2-Cyclohexen-1-ol
0.055
2-cyclohexen-1-one
pH 7.5, 25°C, recombinant enzyme
2.5
cyclohexenone
-
-
4.2
NADH
-
-
0.013
NADPH
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.94 - 10.22
2-Cyclohexen-1-ol
14
trans-hex-2-enal
Solanum lycopersicum
Q9FEW9
-
additional information
additional information
Solanum lycopersicum
Q9FEW9
for oxidative half-reaction, kox of trans-hex-2-2enal is 0.3 per sec
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 8
-
-
7.5
-
assay at
7.5 - 8
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OPRII
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
-
5 - 9
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-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
abundant expression
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
abundant expression
Manually annotated by BRENDA team
-
abundant expression
Manually annotated by BRENDA team
-
abundant expression
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40300
-
calculated from cDNA
41000
x * 41000, recombinant His-tagged enzyme, SDS-PAGE
41100
-
2 * 41100
41170
predicted from amino acid sequence
42000
-
OPRI, gel filtration
42500
-
OPRII, gel filtration
54000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 41000, recombinant His-tagged enzyme, SDS-PAGE
homodimer
-
2 * 41100
monomer
additional information
rapid monomer-dimer equilibrium with a high dissociation constant in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement
-
crystal structure of Arabidopsis thaliana OPR3 in complex with 8-iso prostaglandin A1 (8-iso PGA1) is reported. The crystal structure is solved to 2.6 A. The positioning of 8-iso PGA1 reveals a new binding orientation for substrate in the active site that likely contributes to the relaxed stereospecificity observed for AtOPR3 relative to other OPR's
-
one enzyme monomer per asymmetric unit, space group C2221. Enzyme is a member of an alpha/beta barrel fold family of FMN-containing oxidoreductases
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at 2.0 A resolution, determination of the crystal structures of OPR3 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Phe74 and His244 in OPR3 are critical for substrate filtering; OPR1:p-hydroxybenzaldehyde complex at 2.3 A resolution, determination of the crystal structures of OPR1 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Tyr78 and Tyr246 in OPR1 are critical for substrate filtering
-
native enzyme and mutants E291L and Y364F. Wild-type enzyme crystallizes as an extraordinary self-inhibiting dimer, dimerization is actively driven by the mutual binding of the two L6 loops into the two active sites
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography; by affinity chromatography; by affinity chromatography
OPR1, OPR2 and OPR3
-
purified in enzymatically active form
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography
recombinant isozyme from Escherichia coli
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography
-
recombinant protein
-
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
c-DNA library, gene OPR3 identified
-
cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein
cloned and expressed in Escherichia coli
-
cloning and expression of a gene encoding old yellow enzyme
-
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein
-
construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3); construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3)
enzyme cloned from its cDNA, open reading frame encodes a 372 amino acids polypeptide, functionally expressed in Escherichia coli
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain EHA105
-
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain GV3101
-
expressed in Escherichia coli
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study; expression of His-tagged isozyme OPR2 in Escherichia coli; expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli
-
generating of a jasmonic acid-deficient rice line with suppressed expression of the gene encoding OPR3 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea; generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding OPR1 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases
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OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana
-
OPR3 mutant enzymes are expressed in Escherichia coli
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recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1; recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2; recombinant expression of OPR3 in Escherichia coli
subcloning of the OPR genes from a lambdaGEM11 genomic library
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
up-regulated expression in response to drought, hydrogen peroxide and wounding. Accumulation of HvOPR1 mRNA is increased in response to jasmonic acid, salicylic acid and abscisic acid
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E291L
sixfold faster turnover than wild-type. Crystallization in same space group as wild-type, but with strikingly different cell constants. Appears as monomer in the crystal
F74Y
-
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
F74Y/H244Y
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OPR3 double-mutant, reduction of (9S,13S)-12-oxo-phytodienoic acid is slower in the double-mutant as in the wild type enzyme and the single mutants, the protein crystallizes as a monomer with none of the dimer interactions retain
H244Y
-
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
Y364F
crystallization as a monomer with none of the dimer interactions retained
additional information
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construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
environmental protection
2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants; 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants; 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants
Show AA Sequence (211 entries)
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