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Literature summary for 1.3.1.42 extracted from
- Structural basis of substrate specificity of plant 12-oxophytodienoate reductases (2009), J. Mol. Biol., 392, 1266-1277.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| OPR3 mutant enzymes are expressed in Escherichia coli | Solanum lycopersicum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| at 2.0 A resolution, determination of the crystal structures of OPR3 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Phe74 and His244 in OPR3 are critical for substrate filtering | Solanum lycopersicum |
| OPR1:p-hydroxybenzaldehyde complex at 2.3 A resolution, determination of the crystal structures of OPR1 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Tyr78 and Tyr246 in OPR1 are critical for substrate filtering | Solanum lycopersicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F74Y | OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme | Solanum lycopersicum |
| F74Y/H244Y | OPR3 double-mutant, reduction of (9S,13S)-12-oxo-phytodienoic acid is slower in the double-mutant as in the wild type enzyme and the single mutants, the protein crystallizes as a monomer with none of the dimer interactions retain | Solanum lycopersicum |
| H244Y | OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-Hydroxybenzaldehyde | - |
Solanum lycopersicum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9S,13S)-12-oxo-phytodienoic acid + NADPH + H+ | Solanum lycopersicum | enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| (9S,13S)-12-oxo-phytodienoic acid + NADPH + H+ | Solanum lycopersicum | FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae | ? | - |
? | |
| 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | Solanum lycopersicum | in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae | ? | - |
? | |
| additional information | Solanum lycopersicum | OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | Q9FEW9 | - |
- |
| Solanum lycopersicum | Q9XG54 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9S,13S)-12-oxo-phytodienoic acid + NADPH + H+ | enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid | Solanum lycopersicum | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| (9S,13S)-12-oxo-phytodienoic acid + NADPH + H+ | FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae | Solanum lycopersicum | ? | - |
? | |
| 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae | Solanum lycopersicum | ? | - |
? | |
| additional information | OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate | Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | OPR3 crystallizes as a self-inhibited dimer | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12-oxophytodienoate reductase | - |
Solanum lycopersicum |
| 12-oxophytodienoate reductase 3 | - |
Solanum lycopersicum |
| OPR1 | - |
Solanum lycopersicum |
| OPR3 | - |
Solanum lycopersicum |
| phytodienoic acid reductase | - |
Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Solanum lycopersicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Solanum lycopersicum | |
| NADP+ | - |
Solanum lycopersicum | |
| NADPH | - |
Solanum lycopersicum | |
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