Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,4,6-trinitrotoluene | expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 17fold up-regulation of OPR1 in the root | Arabidopsis thaliana | |
2,4,6-trinitrotoluene | expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 6fold up-regulation of OPR2 in the root | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
environmental protection | 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1 | Arabidopsis thaliana |
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2 | Arabidopsis thaliana |
recombinant expression of OPR3 in Escherichia coli | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | OPR3 | Arabidopsis thaliana | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-12-oxophytodienoic acid + NADPH + H+ | Arabidopsis thaliana | OPR3 is involved in jasmonic acid biosynthesis | 3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GYB8 | ecotypes Col-0 and Wassilewskija | - |
Arabidopsis thaliana | Q8LAH7 | ecotypes Col-0 and Wassilewskija | - |
Arabidopsis thaliana | Q9FUP0 | ecotypes Col-0 and Wassilewskija | - |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
seedling | OPR overexpressing line | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake | Arabidopsis thaliana |
additional information | - |
OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-12-oxophytodienoic acid + NADPH + H+ | OPR3 is involved in jasmonic acid biosynthesis | Arabidopsis thaliana | 3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
additional information | OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes | Arabidopsis thaliana | ? | - |
? | |
additional information | OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives | Arabidopsis thaliana | ? | - |
? | |
additional information | OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPR | - |
Arabidopsis thaliana |
OPR1 | - |
Arabidopsis thaliana |
OPR2 | - |
Arabidopsis thaliana |
OPR3 | - |
Arabidopsis thaliana |
oxophytodienoate reductase | - |
Arabidopsis thaliana |
phytodienoic acid reductase | - |
Arabidopsis thaliana |