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Literature summary for 1.3.1.42 extracted from
- Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products (2004), Mol. Genet. Genomics, 271, 1-10.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | isozyme OPR1 is induced by coronatine | Pisum sativum | |
| additional information | isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine | Pisum sativum | |
| additional information | isozyme OPR4 is induced by coronatine | Pisum sativum | |
| additional information | isozyme OPR6 is induced by coronatine | Pisum sativum | |
| additional information | isozymes OPR3 is slightly induced by coronatine | Pisum sativum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3) | Pisum sativum |
| construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3) | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pisum sativum | isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis | ? | - |
? |  |
| additional information | Pisum sativum | isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | Q76FR7 | isozyme OPR6; 6 isozymes of enzyme family subgroup I | - |
| Pisum sativum | Q76FR8 | isozyme OPR5; 6 isozymes of enzyme family subgroup I | - |
| Pisum sativum | Q76FR9 | isozyme OPR4; 6 isozymes of enzyme family subgroup I | - |
| Pisum sativum | Q76FS0 | isozyme OPR3; 6 isozymes of enzyme family subgroup I | - |
| Pisum sativum | Q76FS1 | isozyme OPR1; 6 isozymes of enzyme family subgroup I | - |
| Pisum sativum | Q9AVK9 | isozyme OPR2; 6 isozymes of enzyme family subgroup I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme from Escherichia coli | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epicotyl | - |
Pisum sativum | - |
| leaf | - |
Pisum sativum | - |
| additional information | isozyme gene expression profiles | Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-cyclohexen-1-one + NADPH | model substrate, isozyme OPR3 | Pisum sativum | ? + NADP+ | - |
? | |
| 2-cyclohexen-1-one + NADPH | model substrate, isozyme OPR4 | Pisum sativum | ? + NADP+ | - |
? | |
| 2-cyclohexen-1-one + NADPH | model substrate, isozyme OPR6 | Pisum sativum | ? + NADP+ | - |
? | |
| 2-cyclohexen-1-one + NADPH | model substrate, isozymes OPR1 | Pisum sativum | ? + NADP+ | - |
? | |
| 2-cyclohexen-1-one + NADPH | model substrate, isozymes OPR2 | Pisum sativum | ? + NADP+ | - |
? | |
| additional information | isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis | Pisum sativum | ? | - |
? | |
| additional information | isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis | Pisum sativum | ? | - |
? | |
| additional information | isozyme OPR5 is inactive | Pisum sativum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | isozymes belong to subgroup I of the 12-oxophytodienoate reductase enzyme family | Pisum sativum |
| OPR1 | - |
Pisum sativum |
| OPR2 | - |
Pisum sativum |
| OPR3 | - |
Pisum sativum |
| OPR4 | - |
Pisum sativum |
| OPR5 | - |
Pisum sativum |
| OPR6 | - |
Pisum sativum |
| oxophytodienoic acid reductase | - |
Pisum sativum |
| S64 | i.e. isozyme PsOPR2 | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Pisum sativum | |
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