2.7.7.47: streptomycin 3''-adenylyltransferase

This is an abbreviated version!
For detailed information about streptomycin 3''-adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.47

2.7.7.47

integrons

medicine

aminoglycoside-modifying

blavim-2

streptomycin-resistant

dfra15

diagnostics

agriculture

analysis

Reaction

Synonyms

AAD (3''), aadA, AadA6, aminoglycoside (3'')(9) adenylyltransferase, aminoglycoside 3''-adenylyltransferase, aminoglycoside 3'-adenyltransferase, aminoglycoside adenyltransferase, aminoglycoside adenyltransferase A, aminoglycoside adenyltransferase type A, aminoglycoside adenylyltransferase, ANT(3'')-II, ANT(3'')-Ii/AAC(6')-IId, SMATase, str, streptomycin adenylate synthetase, streptomycin adenyltransferase, streptomycin adenylylase, streptomycin adenylyltransferase, streptomycin-spectinomycin adenylyltransferase

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.7 Nucleotidyltransferases

2.7.7.47 streptomycin 3''-adenylyltransferase

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Results	in table
2	Activating Compound
2172	AA Sequence
8	Application
1	CAS Registry Number
17	Cloned(Commentary)
3	Cofactor
4	Crystallization (Commentary)
25	General Information
1	General Stability
13	Inhibitors
6	kcat/KM [mM/s]
6	Ki Value [mM]
11	KM Value [mM]
4	Localization
7	Metals/Ions
4	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
57	Organism
12	PDB ID
5	pH Optimum
4	pI Value
17	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
53	Reference
3	Specific Activity [micromol/min/mg]
79	Substrates and Products (Substrate)
7	Subunits
61	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
3	Temperature Stability [°C]
9	Turnover Number [1/s]

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Cloned on EC 2.7.7.47 - streptomycin 3''-adenylyltransferase

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27.8 kb R-plasmid pTET3 gene cassette aadA9, antibiotic resistance region identified in Mycobacterium fortuitum, cloned in Escherichia coli DH5alphaMCR

Corynebacterium glutamicum

643347

aadA resistence gene transferred vi filter mating from Enterococcus faecalis W4770 to Enterococcus faecalis JH2-2, PCR amplified aadA gene cloned into vector pCRII and transformed into Escherichia coli DH5-alpha

Enterococcus faecalis

Q71UU1

643346

cloned in Escherichia coli BL21

Serratia marcescens

672194

expressed in Escherichia coli DH5alpha cells

7 entries

expression in Escherichia coli

Pseudomonas aeruginosa

Q9RGC2

739973

gene aadA2B isolated from integron InC as a gene cassette, Escherichia coli cells transformed by electroporation

Pseudomonas aeruginosa

Q59694

643345

into the vector pGEM-T for sequencing

2 entries

streptomycin adenylyltransferase gene isolated from unconjugative plasmids of Enterococcus casseliflavus HZ95 is cloned in Escherichia coli DH5alpha

Enterococcus casseliflavus

Q58M14

675323

the plastid expression vector pPRV111A carrying the aadA gene encoding aminoglycoside 3''-adenylyltransferase is used for plastid transformation in Solanum melongena

Escherichia coli

706857

thioredoxin-His6-tagged SMATase fusion protein is produced in a bacterial intracellular expression system mainly in a soluble form

Escherichia coli

663311