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Literature summary for 2.7.7.47 extracted from

  • Papadovasilaki, M.; Oberthür, D.; Gessmann, R.; Sarrou, I.; Betzel, C.; Scoulica, E.Petratos, K.
    Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa (2015), Biochem. Biophys. Rep., 4, 152-157.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure, and ternary complex with ATP and streptomycin or spectinomycin Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information generation of a carboxy-terminal truncated variant molecule, residues 1-264. The truncated residues have little influence on protein folding, whereas they have a positive effect on the enzymic activity and stabilize dimers at high protein concentrations Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
kanamycin A competitive Pseudomonas aeruginosa
streptomycin strong substrate inhibition at 1mM MgCl2 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.0004
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
0.0018
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
0.0033
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.0105
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.011
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 required, at least 5 mM Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9RGC2
-
-
Pseudomonas aeruginosa Ps100 Q9RGC2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + spectinomycin
-
Pseudomonas aeruginosa diphosphate + adenylylspectinomycin
-
?
ATP + spectinomycin
-
Pseudomonas aeruginosa Ps100 diphosphate + adenylylspectinomycin
-
?
ATP + streptomycin
-
Pseudomonas aeruginosa diphosphate + adenylylstreptomycin
-
?
ATP + streptomycin
-
Pseudomonas aeruginosa Ps100 diphosphate + adenylylstreptomycin
-
?
additional information no substrates: kanamycin A, gentamycin, and amikacin Pseudomonas aeruginosa ?
-
?
additional information no substrates: kanamycin A, gentamycin, and amikacin Pseudomonas aeruginosa Ps100 ?
-
?

Subunits

Subunits Comment Organism
dimer and monomer, dynamic light scattering Pseudomonas aeruginosa
monomer and dimer, dynamic light scattering Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
AadA6
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49.5
-
melting temperature, wild-type Pseudomonas aeruginosa
51
-
melting temperature, C-terminal deletion mutant Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.41
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.47
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
0.61
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
0.63
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
0.66
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
39
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
55
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
140
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
370
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
1300
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
1600
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa