Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure, and ternary complex with ATP and streptomycin or spectinomycin | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a carboxy-terminal truncated variant molecule, residues 1-264. The truncated residues have little influence on protein folding, whereas they have a positive effect on the enzymic activity and stabilize dimers at high protein concentrations | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kanamycin A | competitive | Pseudomonas aeruginosa | |
streptomycin | strong substrate inhibition at 1mM MgCl2 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.0004 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.0018 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.0033 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.0105 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.011 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | required, at least 5 mM | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9RGC2 | - |
- |
Pseudomonas aeruginosa Ps100 | Q9RGC2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + spectinomycin | - |
Pseudomonas aeruginosa | diphosphate + adenylylspectinomycin | - |
? | |
ATP + spectinomycin | - |
Pseudomonas aeruginosa Ps100 | diphosphate + adenylylspectinomycin | - |
? | |
ATP + streptomycin | - |
Pseudomonas aeruginosa | diphosphate + adenylylstreptomycin | - |
? | |
ATP + streptomycin | - |
Pseudomonas aeruginosa Ps100 | diphosphate + adenylylstreptomycin | - |
? | |
additional information | no substrates: kanamycin A, gentamycin, and amikacin | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no substrates: kanamycin A, gentamycin, and amikacin | Pseudomonas aeruginosa Ps100 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | and monomer, dynamic light scattering | Pseudomonas aeruginosa |
monomer | and dimer, dynamic light scattering | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
AadA6 | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49.5 | - |
melting temperature, wild-type | Pseudomonas aeruginosa |
51 | - |
melting temperature, C-terminal deletion mutant | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.41 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.47 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.61 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.63 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
0.66 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
55 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
140 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
370 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
1300 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
1600 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |