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2.7.7.4: sulfate adenylyltransferase

This is an abbreviated version!
For detailed information about sulfate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.4

Reaction

ATP
+
sulfate
=
diphosphate
 +
adenylyl sulfate

Synonyms

3'-phospho-adenosine-5'-phosphosulphate synthase, 3'-phosphoadenosine-5'-phosphosulfate synthase, AcATPS1, adenosine 5'-triphosphate sulphurylase, adenosine 5'-triphosphate-sulfurylase, adenosine triphosphate sulfurylase, adenosine triphosphate sulphurylase, adenosine triphosphate-sulphurylase, adenosine-5'-triphosphate sulfurylase, adenosinetriphosphate sulfurylase, adenylsulfurylase, adenylylsulfate pyrophosphorylase, adenylyltransferase, sulfate, APS1, APS2, At3g22890, ATP sulfurylase, ATP sulfurylase 1, ATP sulfurylase 2, ATP sulfurylase 3, ATP sulfurylase 4, ATP sulfurylase isoform 1, ATP sulfurylase-APS kinase, ATP sulphurylase, ATP-S, ATP-sulfurylase, ATP: sulfate adenylyl transferase, ATPS, ATPS-A, ATPS-B, ATPS1, ATPS2, CysD, dissimilatory ATP sulfurylase, MgATP:sulfate adenylyltransferase, MgATP:sulfate adenylyltransferase,, PAPS synthase, PAPS synthase 1, PAPS synthase 2, PAPS synthase1, PAPS synthase2, PAPSS1, PAPSs2, SAT, sulfate adenylyltransferase, sulfurylase, TtATPS, TTHA0666

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.4 sulfate adenylyltransferase

Inhibitors

Inhibitors on EC 2.7.7.4 - sulfate adenylyltransferase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
-
3',5'-adenosine diphosphate
-
-
3'-phosphoadenosine 5'-phosphosulfate
allosteric, binding of the inhibitor to the catalytic site as well as to the allosteric site of the wild type enzyme acts to decrease the degree of cooperativity
3'-phosphoadenosine-5'-phosphate
3'-phosphoadenosine-5'-phosphosulfate
3,5-di-tert-butyl-4-hydroxytoluene
-
-
3-tert-butyl-4-hydroxyanisole
ligand diffuses through the gate formed by residues Glu332, Thr312 and His290 to the outside of enzyme. When the gate opens, the ligand is expelled from active site of enzyme, thereafter the ligand returns when the gate closes and binds itself in another region of active site nearby the zinc atom
5,5'-dithiobis(2-nitrobenzoic acid)
0.05 mM, rapid decrease in activity of wild-type enzyme (t1/2: 20 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min
adenosine 5'-monosulfate
-
-
adenosine 5'-phosphoramidate
-
-
adenosine 5'-phosphosulfate
adenylyl sulfate
APS, binding mode, overview. On the ATP sulfurylase domain that initially produces APS from sulfate and ATP, APS acts as a potent product inhibitor, being competitive with both ATP and sulfate. For the APS kinase domain that phosphorylates APS to PAPS, APS is an uncompetitive substrate inhibitor that can bind both at the ATP/ADP-binding site and the PAPS/APS-binding site; APS, binding mode, overview. On the ATP sulfurylase domain that initially produces APS from sulfate and ATP, APS acts as a potent product inhibitor, being competitive with both ATP and sulfate. For the APS kinase domain that phosphorylates APS to PAPS, APS is an uncompetitive substrate inhibitor that can bind both at the ATP/ADP-binding site and the PAPS/APS-binding site
Ba2+
-
2 mM
beta-fluoro-adenosine 5'-phosphosulfate
-
-
beta-methylene-adenosine 5'-phosphosulfate
-
-
ClO3-
deoxyadenylylsulfate
-
1 mM, in presence of about 20% inhibition
diacetyl
-
significant inhibition in the presence of borate, protection by adenosine 5'-phosphosulfate, ATP or MgATP2- plus nitrate
diphosphate
FSO3-
guanylylsulfate
-
1 mM, in presence of adenylylsulfate about 20% inhibition
inosylylsulfate
-
1 mM, in presence of adenylylsulfate about 20% inhibition
iodoacetic acid
methylene blue
-
inactivated by light in presence of methylene blue, protection by adenosine 5'-phosphosulfate
MgATP2-
molybdate
-
N-Acetylimidazole
-
76% of the original activity can be restored by treatment with hydroxylamine
N-ethylmaleimide
Ni2+
-
2 mM
PCMB
-
5 mM, inhibits reaction with diphosphate and adenylylsulfate
Phenylglyoxal
3 mM, irreversible inactivation of wild-type enzyme and mutant enzyme del396-573, t1/2: 5 min for both forms
phosphate
-
inhibition is enhanced by increasing concentrations of Mg2+
S2O32-
SeO42-
SO32-
-
1 mM, 10-25% inhibition
SO42-
Sulfide
Tetranitromethane
-
partial
thiosulfate
competitive with molybdate and noncompetitive with MgATP
Tris-malic acid-KOH buffer
-
Zn2+
-
inhibitory effect even at concentrations as low as 40 mg/l