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2.7.7.4: sulfate adenylyltransferase

This is an abbreviated version!
For detailed information about sulfate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.4

Reaction

ATP
+
sulfate
=
diphosphate
 +
adenylyl sulfate

Synonyms

3'-phospho-adenosine-5'-phosphosulphate synthase, 3'-phosphoadenosine-5'-phosphosulfate synthase, AcATPS1, adenosine 5'-triphosphate sulphurylase, adenosine 5'-triphosphate-sulfurylase, adenosine triphosphate sulfurylase, adenosine triphosphate sulphurylase, adenosine triphosphate-sulphurylase, adenosine-5'-triphosphate sulfurylase, adenosinetriphosphate sulfurylase, adenylsulfurylase, adenylylsulfate pyrophosphorylase, adenylyltransferase, sulfate, APS1, APS2, At3g22890, ATP sulfurylase, ATP sulfurylase 1, ATP sulfurylase 2, ATP sulfurylase 3, ATP sulfurylase 4, ATP sulfurylase isoform 1, ATP sulfurylase-APS kinase, ATP sulphurylase, ATP-S, ATP-sulfurylase, ATP: sulfate adenylyl transferase, ATPS, ATPS-A, ATPS-B, ATPS1, ATPS2, CysD, dissimilatory ATP sulfurylase, MgATP:sulfate adenylyltransferase, MgATP:sulfate adenylyltransferase,, PAPS synthase, PAPS synthase 1, PAPS synthase 2, PAPS synthase1, PAPS synthase2, PAPSS1, PAPSs2, SAT, sulfate adenylyltransferase, sulfurylase, TtATPS, TTHA0666

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.4 sulfate adenylyltransferase

Cloned

Cloned on EC 2.7.7.4 - sulfate adenylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Aquifex aeolicus ATP-sulfurylase-APS kinase is expressed in an Escherichia coli pET vector expression system
-
ATP sulfurylase cDNA from MET3 on chromosome X is amplified and expressed in Escherichia coli XL1-Blue. The synthesis of the enzyme is directed by an expression system that employs the regulatory genes of Vibrio fischeri
-
bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase. Full-length enzyme and its constituent adenosine 5'-phosphosulfate kinase and ATP sulfurylase domains are individually expressed
-
bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase. Full-length enzyme and its constituent adenosine 5'-phosphosulfate kinase and ATP sulfurylase domains are individually expressed and purified. Expressed protein generated from the ATP-sulfurylase domain alone is fully active in both the forward and the reverse assays. APS kinase-only recombinants exhibit no kinase activity
-
cloned in Escherichia coli
cloned in Escherichia coli as a truncated version of soybean ATPS lacking the first 48 amino acids, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli
cloned in Escherichia coli BL-21
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21-DE3
expressed in Escherichia coli BL21Star (DE3) cells
-
expression in Escherichia coli BL-21
gene APS2 or At1g19920, Arabidopsis thaliana APS isozyme sequence comparisons, protoplast isolation and transfection of APS2, expression of GFP(S65T)-tagged enzyme under control of CaMV 35S promoter, the binary plasmids are transferred to Agrobacteriumtume faciens C58C1 GV3101 that are transformed by floral dip method into plants. Translationof ATPS2 mRNA starts at multiple sites, AUGMet1 and eitherAUGMet52 or AUGMet58, to produce the plastidic and the cytosolic ATPS2 isoforms, respectively, in Arabidopsis thaliana. The alternative translational initiation sites of ATPS2 are determined by expression of dual-luciferase-tagged fusion constructs of wild-type and mutant isozyme ATPS2 in Arabidopsis protoplasts. The translation of chloroplastic ATPS2 pre-protein can only be initiated from the AUGMet1 start codon
gene atps1 or At3g22890, examination of ATPS1 sequences of varieties Bay-0 and Shahdara identifying two deletions in the first intron and immediately downstream the gene in Bay-0 shared with multiple other Arabidopsis accessions. The average ATPS1 transcript levels are lower in these accessions than in those without the deletions, while sulfate levels are significantly higher
gene sat, sequence comparisons, the sat gene is located in one operon and co-transcribed with the aprMBA genes for membrane-bound APS reductase, recombinant expression of His-tagged enzyme in Escherichia coli
His-tag version expressed in Escherichia coli BL21-DE3
isoform PAPS synthase 1 wild type and mutant proteins are expressed in Escherichia coli
overexpression in Eacherichia coli
-
recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3)
sequence comparisons and phylogenetic tree, recombinant expression of wild-type and mutant N-terminally truncated and His6-tagged enzymes in Escherichia coli strain Rosetta (DE3)
the four ATP-S genes ATPS1,-2,-3, and -4 have N-terminal extensions typ ical of plastid-transit peptides, and are located on different chromosomes
the four ATP-S genes ATPS1,-2,-3, and -4 have N-terminal extensions typical of plastid-transit peptides, and are located on different chromosomes
the protein is insoluble when the full-length cDNA of soybean ATPS, including the chloroplast/plastid localisation sequence, is used for expression in Escherichia coli
truncated enzyme form