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Literature summary for 2.7.7.4 extracted from
- Complex formation between recombinant ATP sulfurylase and APS reductase of Allium cepa (L.) (2007), FEBS Lett., 581, 4139-4147.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine-5'-phosphosulfate reductase 1 | slight stimulation of ATPS1 activity is noted with the addition of the 5fold volume of full-length adenosine-5'-phosphosulfate reductase 1 | Allium cepa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Allium cepa |
| expressed in Escherichia coli BL21 cells | Allium cepa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Allium cepa | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Allium cepa |
| 50000 | - |
SDS-PAGE and gel filtration | Allium cepa |
| 51810 | - |
calculated from sequence of cDNA | Allium cepa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium cepa | - |
- |
- |
| Allium cepa | Q9SDP4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed enzyme is purified by affinity chromatography using glutathione-Sepharose and subsequent ion-exchange chromatography | Allium cepa |
| glutathione-Sepharose column chromatography and Mono Q column chromatography | Allium cepa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.98 | - |
ratio AcATPS1-AcAPR1 1:1 | Allium cepa |
| 12.96 | - |
ratio AcATPS1-AcAPR1 1:0 | Allium cepa |
| 12.96 | - |
recombinant enzyme, at 25°C | Allium cepa |
| 15.25 | - |
ratio AcATPS1-AcAPR1 1:5, five-fold excess of AcAPR1 slightly increases the activity of AcATPS1 | Allium cepa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Allium cepa | diphosphate + adenylylsulfate | - |
? |  |
| ATP + sulfate | it is shown that AcATPS1 and adenosine-5'-phopshosulfate reductase (AcAPR1) from Allium cepa form protein-protein complexes in vitro, thereby a slight stimulation AcATPS1 activity is detectable | Allium cepa | diphosphate + adenylyl sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcATPS1 | - |
Allium cepa |
| ATP sulfurylase | - |
Allium cepa |
| ATPS1 | - |
Allium cepa |
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