General Stability | Organism |
---|---|
freezing and thawing inactivates the purified enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2 mM | Mus musculus | |
Ca2+ | 2 mM | Mus musculus | |
deoxyadenylylsulfate | 1 mM, in presence of about 20% inhibition | Mus musculus | |
EDTA | inhibition is reversed by mn2+, Mg2+, Cu2+, Co2+ | Mus musculus | |
guanylylsulfate | 1 mM, in presence of adenylylsulfate about 20% inhibition | Mus musculus | |
inosylylsulfate | 1 mM, in presence of adenylylsulfate about 20% inhibition | Mus musculus | |
Ni2+ | 2 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the reaction does not strictly follow Michaelis-Menten kinetics | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus | |
Mn2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus | |
Zn2+ | no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mastocytoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
0-4°C, purified enzyme solution, 1 mg protein per ml, 15% loss of activity after 5 weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Mus musculus | diphosphate + adenylylsulfate | - |
r | |
dATP + SO42- | - |
Mus musculus | diphosphate + deoxyadenylylsulfate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mus musculus |