Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.4 extracted from

  • Renosto, F.; Patel, H.C.; Martin, R.L.; Thomassian, C.; Zimmerman, G.; Segel, I.H.
    ATP sulfurylase from higher plants: kinetic and structural characterization of the chloroplast and cytosol enzymes from spinach leaf (1993), Arch. Biochem. Biophys., 307, 272-285.
    View publication on PubMed
Search for more literature for 2.7.7.4

Inhibitors

Inhibitors Comment Organism Structure
3',5'-adenosine diphosphate
-
 Spinacia oleracea
3'-phosphoadenosine-5'-phosphate
-
 Spinacia oleracea
adenosine 5'-monosulfate
-
 Spinacia oleracea
adenosine 5'-phosphoramidate
-
 Spinacia oleracea
adenosine 5'-phosphosulfate inhibits molybdolysis Spinacia oleracea
ADP
-
 Spinacia oleracea
AMP
-
 Spinacia oleracea
beta-fluoro-adenosine 5'-phosphosulfate
-
 Spinacia oleracea
beta-methylene-adenosine 5'-phosphosulfate
-
 Spinacia oleracea
NAD+
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00053
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
0.0037
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
0.017
-
 diphosphate pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
0.045
-
 ATP pH 8.0, 30°C, chloroplastic enzyme, reaction with MoO42- Spinacia oleracea
0.045
-
 ATP ATP in form of MgATP2- Spinacia oleracea
0.046
-
 ATP pH 8.0, 30°C, chloroplastic enzyme, reaction with SO42- Spinacia oleracea
0.046
-
 ATP ATP in form of MgATP2- Spinacia oleracea
0.1
-
 diphosphate pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
0.15
-
 ATP pH 8.0, 30°C, cytosolic enzyme, reaction with MoO42- Spinacia oleracea
0.15
-
 ATP ATP in form of MgATP2- Spinacia oleracea
0.24
-
 ATP pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
0.24
-
 ATP ATP in form of MgATP2- Spinacia oleracea
0.25
-
 SO42- pH 8.0, 30°C, chloroplastic enzyme, reaction with SO42- Spinacia oleracea
0.32
-
 MoO42- pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
0.36
-
 MoO42- pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
0.5
-
 ATP pH 8.0, 30°C, chloroplastic enzym, reaction with FPO32- Spinacia oleracea
0.5
-
 ATP ATP in form of MgATP2- Spinacia oleracea
0.87
-
 SO42- cytosolic enzyme Spinacia oleracea
0.87
-
 SO42- pH 8.0, 30°C Spinacia oleracea
1.3
-
 FPO32- pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
cytosol
-
 Spinacia oleracea 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 4 * 49000, chloroplastic enzyme, SDS-PAGE Spinacia oleracea
50000
-
 4 * 50000, cytosolic enzyme, SDS-PAGE Spinacia oleracea
170000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a chloroplastic and a cytosolic enzyme form Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
247
-
 cytosolic enzyme Spinacia oleracea
267
-
 chloroplastic enzyme Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + fluorophosphate
-
 Spinacia oleracea diphosphate + adenylyl fluorophosphate
-
 r
ATP + FPO32-
-
 Spinacia oleracea ?
-
 ?
ATP + molybdate
-
 Spinacia oleracea diphosphate + adenylyl molybdate
-
 r
ATP + MoO42-
-
 Spinacia oleracea AMP + adenylylmolybdate
-
 ?
ATP + sulfate
-
 Spinacia oleracea diphosphate + adenylylsulfate
-
 r
ATP + sulfate
-
 Spinacia oleracea diphosphate + adenylyl sulfate
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 49000, chloroplastic enzyme, SDS-PAGE Spinacia oleracea
tetramer 4 * 50000, cytosolic enzyme, SDS-PAGE Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.5 about 90% of maximal activity at pH 7.2 and pH 8.5 Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
0.0025
-
 beta-methylene-adenosine 5'-phosphosulfate pH 8.0, 30°C, ATP synthesis Spinacia oleracea
0.003
-
 beta-methylene-adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
0.0047
-
 beta-fluoro-adenosine 5'-phosphosulfate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
0.005
-
 beta-fluoro-adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
0.087
-
 ADP pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
0.165
-
 AMP pH 8.0, 30°C, synthesis of AMP Spinacia oleracea
0.24
-
 adenosine 5'-phosphoramidate pH 8.0, 30°C, synthesis of AMP Spinacia oleracea
0.25
-
 adenosine 5'-monosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
0.35
-
 3',5'-adenosine diphosphate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
0.42
-
 adenosine 5'-monosulfate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
0.9
-
 NAD+ pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
1.3
-
 3',5'-adenosine diphosphate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.5
-
 NAD+ pH 8.0, 30°C, molybdolysis Spinacia oleracea