2.7.1.29: glycerone kinase
This is an abbreviated version!
For detailed information about glycerone kinase, go to the full flat file.
Word Map on EC 2.7.1.29
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2.7.1.29
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dissimilation
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1,3-propanediol
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freundii
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dhaklm
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phosphoenolpyruvate-dependent
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hemiaminal
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phosphoenolpyruvate:carbohydrate
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biofuel production
- 2.7.1.29
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dissimilation
- 1,3-propanediol
- freundii
- dhaklm
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phosphoenolpyruvate-dependent
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hemiaminal
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phosphoenolpyruvate:carbohydrate
- biofuel production
Reaction
Synonyms
acetol kinase, ATP-dependent dihydroxyacetone kinase, dAK, DHA kinase, DhaK, DhaK-2, DhaKI, DhaL, dihydroxyacetone kinase, dihydroxyacetone kinase I, FMN cyclase/dha kinase, glycerone kinase, kinase, acetol (phosphorylating), More, PEP-dependent Dha kinase, phosphoenolpyruvate carbohydrate phosphotransferase
ECTree
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Engineering
Engineering on EC 2.7.1.29 - glycerone kinase
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E526K
H56A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
H56N
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
additional information
E526K
based on the use of hybrid quantum mechanics/molecular mechanics (QM/MM) potentials, with the QM region described by semiempirical and DFT methods, the reaction mechanism of the wild-type enzyme and the most active experimentally measured mutant (Glu526Lys) with polyphosphate as phosphoryl donor is explored to elucidate the origin of the activity of this mutant. The mutation favors a more adequate position of the polyphosphate in the active site for the following step, the chemical reaction, to take place. Structure-function analysis, overview
constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview
additional information
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constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview
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additional information
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constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview
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additional information
by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
additional information
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by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
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additional information
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by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
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additional information
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by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
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additional information
B9LNV8; B9LNV9
by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
additional information
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by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts
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additional information
1-propanol production from glycerol is achieved by addition of the ATP-dependent dihydroxyacetone kinase gene to Escherichia coli strain BW38029 harboring pKK_mde and pRSF_pduCDEGOQS, reconstruction of the 1,2-propanediol (1,2-PD) synthetic pathway (pKK_mde), pathway overview
additional information
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1-propanol production from glycerol is achieved by addition of the ATP-dependent dihydroxyacetone kinase gene to Escherichia coli strain BW38029 harboring pKK_mde and pRSF_pduCDEGOQS, reconstruction of the 1,2-propanediol (1,2-PD) synthetic pathway (pKK_mde), pathway overview
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