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Literature summary for 2.7.1.29 extracted from

  • Sanchez-Moreno, I.; Bordes, I.; Castillo, R.; Ruiz-Pernia, J.J.; Moliner, V.; Garcia-Junceda, E.
    Tuning the phosphoryl donor specificity of dihydroxyacetone kinase from ATP to inorganic polyphosphate. An insight from computational studies (2015), Int. J. Mol. Sci., 16, 27835-27849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
E526K the mutant shows activity with polyphosphate Citrobacter freundii
K382A the mutant shows activity with polyphosphate Citrobacter freundii
K514A the mutant shows activity with polyphosphate Citrobacter freundii
R207B the mutant shows activity with polyphosphate Citrobacter freundii
R475A the mutant shows activity with polyphosphate Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
ATP at pH 8.0 and 37°C Citrobacter freundii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 63000, SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dihydroxyacetone Citrobacter freundii
-
ADP + dihydroxyacetone phosphate
-
?
ATP + dihydroxyacetone Citrobacter freundii CECT 4626
-
ADP + dihydroxyacetone phosphate
-
?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q1KLC3
-
-
Citrobacter freundii CECT 4626 Q1KLC3
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-iminodiacetic acid agarose column chromatography Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydroxyacetone
-
Citrobacter freundii ADP + dihydroxyacetone phosphate
-
?
ATP + dihydroxyacetone
-
Citrobacter freundii CECT 4626 ADP + dihydroxyacetone phosphate
-
?
additional information the enzyme does not show activity with GTP, CTP, TTP and UTP, inorganic triphosphate, and polyphosphate Citrobacter freundii ?
-
?
additional information the enzyme does not show activity with GTP, CTP, TTP and UTP, inorganic triphosphate, and polyphosphate Citrobacter freundii CECT 4626 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 63000, SDS-PAGE Citrobacter freundii

Synonyms

Synonyms Comment Organism
DhaK
-
Citrobacter freundii
dihydroxyacetone kinase
-
Citrobacter freundii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.02
-
ATP at pH 8.0 and 37°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
69
-
ATP at pH 8.0 and 37°C Citrobacter freundii