Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
E526K | the mutant shows activity with polyphosphate | Citrobacter freundii |
K382A | the mutant shows activity with polyphosphate | Citrobacter freundii |
K514A | the mutant shows activity with polyphosphate | Citrobacter freundii |
R207B | the mutant shows activity with polyphosphate | Citrobacter freundii |
R475A | the mutant shows activity with polyphosphate | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
ATP | at pH 8.0 and 37°C | Citrobacter freundii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, SDS-PAGE | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | Citrobacter freundii | - |
ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | Citrobacter freundii CECT 4626 | - |
ADP + dihydroxyacetone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | Q1KLC3 | - |
- |
Citrobacter freundii CECT 4626 | Q1KLC3 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-iminodiacetic acid agarose column chromatography | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | - |
Citrobacter freundii | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | - |
Citrobacter freundii CECT 4626 | ADP + dihydroxyacetone phosphate | - |
? | |
additional information | the enzyme does not show activity with GTP, CTP, TTP and UTP, inorganic triphosphate, and polyphosphate | Citrobacter freundii | ? | - |
? | |
additional information | the enzyme does not show activity with GTP, CTP, TTP and UTP, inorganic triphosphate, and polyphosphate | Citrobacter freundii CECT 4626 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 63000, SDS-PAGE | Citrobacter freundii |
Synonyms | Comment | Organism |
---|---|---|
DhaK | - |
Citrobacter freundii |
dihydroxyacetone kinase | - |
Citrobacter freundii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.02 | - |
ATP | at pH 8.0 and 37°C | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
ATP | at pH 8.0 and 37°C | Citrobacter freundii |