Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H169A | completely inactive | Escherichia coli |
H439A | completely inactive | Escherichia coli |
H9A | completely inactive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
dihydroxyacetone | pH 7.5, 30°C, DhaK-DhaL-DhaM complex | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P37349 | phosphoryl donor DhaM | - |
Escherichia coli | P76014 | DhaL subunit | - |
Escherichia coli | P76015 | DhaK subunit | - |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, Superdex 75 | Escherichia coli |
HighQ anion exchange, Superdex 200 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylated DhaM domain of dihydroxyacetone kinase + dihydroxyacetone | enzyme complex uses the PEP:sugar phosphotransferase protein DhaM instead of ATP as phosphoryl donor | Escherichia coli | dephospho-DhaM + dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000 | Escherichia coli |
More | approx. 18000 Da, monomeric state is suggested | Escherichia coli |
More | approx. 35000 Da, monomeric state is suggested | Escherichia coli |
More | enzyme comlex is present in an approx. 1/1/1 ratio of DhaK, DhaL and DhaM | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
dihydroxyacetone | pH 7.5, 30°C, DhaK-DhaL-DhaM complex | Escherichia coli |