Information on EC 2.7.1.29 - glycerone kinase

Word Map on EC 2.7.1.29
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.29
-
RECOMMENDED NAME
GeneOntology No.
glycerone kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + glycerone = ADP + glycerone phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
formaldehyde assimilation III (dihydroxyacetone cycle)
-
-
glycerol degradation II
-
-
glycolysis
-
-
Glycerolipid metabolism
-
-
Methane metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:glycerone phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-47-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KD1
-
-
Manually annotated by BRENDA team
strain KD1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
VPI 3266
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Listeria innocua CLIP 11262
-
-
-
Manually annotated by BRENDA team
no activity in Clostridium acetobutylicum
DG1(pSPD5)
-
-
Manually annotated by BRENDA team
no activity in Clostridium acetobutylicum DG1(pSPD5)
DG1(pSPD5)
-
-
Manually annotated by BRENDA team
strain CBS 4732
-
-
Manually annotated by BRENDA team
strain CBS 4732
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + acetol
ADP + acetol phosphate
show the reaction diagram
-
i.e. 1-hydroxy-2-propanone
-
?
ATP + D,L-glyceraldehyde
ADP + D,L-glyceraldehyde 3-phosphate
show the reaction diagram
-
D,L-glyceraldehyde binds strongly to the enzyme, slow product release
-
-
?
ATP + dihydroxyacetone
ADP + dihydroxyacetone phosphate
show the reaction diagram
ATP + DL-glyceraldehyde
ADP + glyceraldehyde 3-phosphate
show the reaction diagram
ATP + glycerone
ADP + glycerone phosphate
show the reaction diagram
CTP + dihydroxyacetone
CDP + dihydroxyacetone phosphate
show the reaction diagram
dihydroxyacetone + ATP
dihydroxyacetone phosphate + ADP
show the reaction diagram
GTP + dihydroxyacetone
GDP + dihydroxyacetone phosphate
show the reaction diagram
ITP + dihydroxyacetone
IDP + dihydroxyacetone phosphate
show the reaction diagram
phospho-DhaM + 3,4-dihydroxy-2-butanone
dephospho-DhaM + 3-hydroxy-2-butanone-4-phosphate
show the reaction diagram
-
-
?
phospho-DhaM + erythrose
dephospho-DhaM + erythrose 4-phosphate
show the reaction diagram
-
-
?
phospho-DhaM + glyceraldehyde
dephospho-DhaM + glyceraldehyde 2-phosphate
show the reaction diagram
-
-
?
phosphoenolpyruvate + D,L-glyceraldehyde
pyruvate + D,L-glyceraldehyde 3-phosphate
show the reaction diagram
-
-
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
show the reaction diagram
phosphorylated DhaM domain of dihydroxyacetone kinase + dihydroxyacetone
dephospho-DhaM + dihydroxyacetone phosphate
show the reaction diagram
enzyme complex uses the PEP:sugar phosphotransferase protein DhaM instead of ATP as phosphoryl donor
-
?
UTP + dihydroxyacetone
UDP + dihydroxyacetone phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dihydroxyacetone
ADP + dihydroxyacetone phosphate
show the reaction diagram
ATP + glycerone
ADP + glycerone phosphate
show the reaction diagram
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no tightly bound cofactor
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
4 mM, 12% activation
additional information
-
no tightly bound metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chloro-3-hydroxyacetone
D,L-glyceraldehyde
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the DhaR transcription factor stimulates the transcription of the dhaKLM operon from a sigma70 promotor and autorepresses expression of DhaR
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.022
1,3-dihydroxy-2-propanone
isoenzyme Dak1
0.1 - 0.63
ATP
0.18
D,L-glyceraldehyde
-
pH 7.5, 30C
0.00122 - 2.2
dihydroxyacetone
0.024
DL-glyceraldehyde
-
pH 7.5, 30C
0.005 - 0.006
glycerone phosphate
0.35
MgATP2-
-
pH 8.0, 30-37C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.17
D,L-glyceraldehyde
Citrobacter freundii
-
pH 7.5, 30C
1.6 - 24.13
dihydroxyacetone
4.8 - 17.5
glycerone phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7 - 19800
dihydroxyacetone
620
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14 - 0.2
ADP
0.15 - 0.6
D,L-glyceraldehyde
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 8.2
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
isoenzyme Dak1, gel filtration
145000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
-
-
trimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of enzyme complex with an ATP analogue and dihydroxyacetone at 2.5 A resolution
apoenzyme, from solution containing 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% w/v PEG 4000, 15% w/v methylpentanediol, hanging drop vapour diffusion method, apoenzyme-crystals are soaked in 2 mM glyceraldehyde or 5 mM dihydroxyacetone phosphate and flash frozen at -168C, X-ray diffraction structure determination and analysis at 2.0 and 1.9 A resolution, respectively
-
crystal structure of the nucleotide-binding subunit DhaL
Q0TIH0
DhaK and DhaK-dihydroxacetone complex are crystallized from 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% polyethylene glycol 4000, 15% 2-methyl-2,4-pentanediol using hanging drop vapor diffusion, crystals diffract to 1.75 A resolution
DhaK-DhaL complex bound to ADP anddihydroxyacetone, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 3.5 M sodium formate or 0.1 M sodium citrate pH 5.6, 20% (w/v)PEG 8000, at 19C
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 8.7
-
optimal stability
641267
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48.7
-
the melting temperature is 48.7C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme-D,L-glyceraldehyde complex is resistant to SDS
-
glycerol, most effective stabilizer, EDTA and MgCl2 stabilize to a lesser extent, inactivated during ammonium sulfate precipitation
-
not affected by 2 M glycerol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 40% glycerol, at least 1 month, no loss of activity
-
-20C, 60% glycerol, at least 4 weeks, no loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, butyl-Toyopearl, UNO-Q6, recombinant enzyme
DEAE-cellulose, ResourceQ, Superdex 200
DEAE-cellulose, Superdex 75; DEAE-cellulose, Superdex 75; HighQ anion exchange, Superdex 200
HisTrap column chromatography
-
Ni+2/IDA agarose column chromatography and Superdex 75 gel filtration
-
Ni-NTA affinity column chromatography, gel filtration
-
nickel affinity column chromatography and Superdex 200 gel filtration
-
polyethylene glycol 6000, polyethyleneimine, DEAE-Sepharose, Sepharose CL-6B
-
streptomycin sulfate, ammonium sulfate, Cibacron blue F3G-ASephadex g-200, DEAE cellulose, partially purified
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli M15pREP4 cells
-
expressed in Escherichia coli NM522 cells
expressed in Escherichia coli strain BL21(DE3) and in chloroplasts from Nicotiana tabacum cultivar Xanthi
-
expression in BL21 cells
expression of dak 1 in Escherichia coli
overexpression in Escherichia coli
overexpression in Escherichia coli; overexpression in Escherichia coli; overexpression in Escherichia coli
phylogenetic analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
inactivation of the gene lin0370 causes 20fold-elevated expression level of the dhaK-2 gene
the enzyme has nearly no activity changes under continuous cultivation from 0.5 to 3.0 M NaCl but the activity increases significantly with further increased salinities and achieves the maximum under 4.5 M NaCl
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D109A
-
inactive
D109N
-
inactive
H128A
-
inactive
H128K
-
inactive
H169A
completely inactive
H439A
completely inactive
H56A
-
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
H56N
-
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
H9A
completely inactive
Show AA Sequence (936 entries)
Please use the Sequence Search for a certain query.