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2.7.1.176: UDP-N-acetylglucosamine kinase

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.176

Reaction

ATP
+
UDP-N-acetyl-alpha-D-glucosamine
=
ADP
+
UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate

Synonyms

epsilon/zeta toxin-antitoxin homologue, Ngzeta_1, ng_epsilon1 / ng_zeta1, ng_zeta1 toxin, PezT, TA homologue, toxin PezT, toxin zeta, UNAG kinase, zeta toxin, Zeta-toxin

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.176 UDP-N-acetylglucosamine kinase

Purification

Purification on EC 2.7.1.176 - UDP-N-acetylglucosamine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant fused His-tagged PezT with His-tagged PezA from Escherichia coli BL21(DE3) by nickel affinity chromatography, PezA is cleaved off by thrombin, followed by anion exchange and gel filtration to purifiy PezT and PezA, quantitative real-time RT PCR expression analysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIL by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes with or without PezA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant truncated variant PezTDELTAC242 from Escherichia coli strain BL21(DE3)