2.7.1.176: UDP-N-acetylglucosamine kinase

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.176

2.7.1.176

pyogenes

streptococcal

omega

stasis

low-copy-number

peptidoglycan

poisons

addiction

erythromycin

secure

biotechnology

Reaction

UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate

Synonyms

epsilon/zeta toxin-antitoxin homologue, Ngzeta_1, ng_epsilon1 / ng_zeta1, ng_zeta1 toxin, PezT, TA homologue, toxin PezT, toxin zeta, UNAG kinase, zeta toxin, Zeta-toxin

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.176 UDP-N-acetylglucosamine kinase

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Results	in table
2	Activating Compound
1576	AA Sequence
1	Application
10	Cloned(Commentary)
7	Cofactor
3	Crystallization (Commentary)
22	General Information
1	General Stability
1	Inhibitors
6	kcat/KM [mM/s]
8	KM Value [mM]
5	Metals/Ions
6	Natural Substrates/ Products (Substrates)
12	Organism
1	pH Optimum
26	Protein Variants
6	Purification (Commentary)
1	Reaction
11	Reference
14	Substrates and Products (Substrate)
1	Subunits
24	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
6	Turnover Number [1/s]

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Cloned on EC 2.7.1.176 - UDP-N-acetylglucosamine kinase

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DNA and amino acid sequence comparisons

Streptococcus pyogenes

Q54944

718312

expression of His-tagged wild-type and mutant enzymes with or without PezA in Escherichia coli strain BL21(DE3)

Streptococcus pneumoniae

717793

expression of zeta toxin in Escherichia coli under control of the LacI repressor-Hyper-Spank promoter using vector pDR111

Bacillus subtilis

718300

gene pezT, DNA and amino acid sequence determination and analysis, expression of truncated variant PezTDELTAC242 in Escherichia coli strain BL21(DE3)

3 entries

gene pezT, DNA and amino acid sequence determination and analysis, sequence comparison, co-expression of fused His-tagged PezT with His-tagged PezA in Escherichia coli BL21(DE3). The PezA antitoxin represses transcription from PpezAT and the PezT toxin acts as a co-repressor

Streptococcus pneumoniae

Q97QZ1

717773

gene pezT, recombinant expression of GFP-tagged enzyme in the eukaryotic microalga Chlorella vulgaris strain UMT-M1, Agrobacterium tumefaciens strain LBA4404-mediated co-transformation with recombinant activator and responder vectors, is lethal. Following induction by 17-beta-estradiol for the expression of the toxin-GFP fusion transgenes, algal cells show signs of cellular damage and lysis. Vector construct overview

Streptococcus pneumoniae

Q97QZ1

737412

gene pezT, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-RIL, Ngzeta_1 expression in Escherichia coli cells causes a lytic phenotype compensated by ngepsilon_1 coexpression. Growth curves of Escherichia coli C41(DE3) cells expressing ngzeta_1 wild-type and ngzeta_1 mutant K115A show that when ngzeta_1 is expressed in Escherichia coli cells, a strong lytic phenotype 30 min post-induction occurs

Neisseria gonorrhoeae

A0A5K1QBJ4

761997

the gene is encoded in plasmid pSM19035. The omega-epsilon-zeta operon of this plasmid constitutes a proteic plasmid addiction system in which the epsilon and zeta genes encode an antitoxin and toxin, respectively, while omega plays an autoregulatory function

Streptococcus pyogenes

Q54944

717722