2.7.1.176: UDP-N-acetylglucosamine kinase
This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine kinase, go to the full flat file.
Word Map on EC 2.7.1.176
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2.7.1.176
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pyogenes
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streptococcal
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omega
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stasis
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low-copy-number
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peptidoglycan
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poisons
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addiction
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erythromycin
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secure
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biotechnology
- 2.7.1.176
- pyogenes
- streptococcal
- omega
-
stasis
-
low-copy-number
- peptidoglycan
-
poisons
-
addiction
- erythromycin
-
secure
- biotechnology
Reaction
Synonyms
epsilon/zeta toxin-antitoxin homologue, Ngzeta_1, ng_epsilon1 / ng_zeta1, ng_zeta1 toxin, PezT, TA homologue, toxin PezT, toxin zeta, UNAG kinase, zeta toxin, Zeta-toxin
ECTree
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Engineering
Engineering on EC 2.7.1.176 - UDP-N-acetylglucosamine kinase
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Y83C
D66T
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site-directed mutagenesis, a nontoxicPezT variant, no bulge formation or lysis after induction of PezT D66T
D66T
D66T/W232Y
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site-directed mutagenesis, a mutated tryptophan-free PezT variant
K45A
site-directed mutagenesis, the mutation abolishes PezT lethality
R157A
site-directed mutagenesis, the mutation abolishes PezT lethality
R170A
site-directed mutagenesis, the mutation abolishes PezT lethality
T117V
site-directed mutagenesis, the mutation abolishes PezT lethality
T120V
site-directed mutagenesis, the mutation abolishes PezT lethality and a delay in the growth inhibition
A111C
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one
A248G
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one
D66T
site-directed mutagenesis, a nontoxicPezT variant, no bulge formation or lysis after induction of PezT D66T
K46A
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon
K46A/D67T
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon
R158A
site-directed mutagenesis, the mutant construct can be cloned into Escherichia coli but not overexpressed
R158A/R171S
site-directed mutagenesis, the mutant can be cloned and expressed
additional information
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site-directed mutagenesis, the mutant induces the dormant state with equal efficiency as the wild-type toxin independently of the KCl concentration of 5-150 mM. zetaY83C-induced dormancy is characterized by minimal metabolism, with the repression of genes involved in glycolysis such as yqeC, mtlD, glpK, glpD, yvkC, gutP, gntK, gntR, and gntZ. zetaY83C toxin induction also affects ATP and GTP production levels, overview
Y83C
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site-directed mutagenesis, the mutant induces the dormant state with equal efficiency as the wild-type toxin independently of the KCl concentration of 5-150 mM. zetaY83C-induced dormancy is characterized by minimal metabolism, with the repression of genes involved in glycolysis such as yqeC, mtlD, glpK, glpD, yvkC, gutP, gntK, gntR, and gntZ. zetaY83C toxin induction also affects ATP and GTP production levels, overview
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D66T
site-directed mutagenesis, a nontoxicPezT variant, no bulge formation or lysis after induction of PezT D66T
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construction of truncated variant PezTDELTAC242 , the variant does not accumulate any spontaneous mutations and still retains the toxic phenotype
additional information
construction of truncated variant PezTDELTAC242 , the variant does not accumulate any spontaneous mutations and still retains the toxic phenotype
additional information
a mutant variant truncated after Val-234 lacks the helix K-loop-helix L appendage
additional information
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a mutant variant truncated after Val-234 lacks the helix K-loop-helix L appendage
additional information
construction of truncated variant PezTDELTAC242 , the variant does not accumulate any spontaneous mutations and still retains the toxic phenotype