2.7.1.161: CTP-dependent riboflavin kinase
This is an abbreviated version!
For detailed information about CTP-dependent riboflavin kinase, go to the full flat file.
Reaction
Synonyms
CTP-dependent riboflavin kinase, Methanocaldococcus jannaschii Mj0056, MJ0056, myRbkR, pyRbkR, RbkR, RibK, riboflavin kinase, taRbkR
ECTree
Advanced search results
Purification
Purification on EC 2.7.1.161 - CTP-dependent riboflavin kinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2(DE3)pLysS by nickel affinity chromatography, gel filtration, hydrophobic interaction chhromatography, and ultrafiltration
recombinant N-terminally His6-Smt3-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His6-Smt3-tag is then cleaved from the purified RbkR protein by digestion with Ulp1 protease
recombinant N-terminally His6-Smt3-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His6-Smt3-tag is then cleaved from the purified RbkR protein by digestion with Ulp1 protease