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2.7.1.161: CTP-dependent riboflavin kinase

This is an abbreviated version!
For detailed information about CTP-dependent riboflavin kinase, go to the full flat file.

Reaction

CTP
+
riboflavin
=
CDP
+
FMN

Synonyms

CTP-dependent riboflavin kinase, Methanocaldococcus jannaschii Mj0056, MJ0056, myRbkR, pyRbkR, RbkR, RibK, riboflavin kinase, taRbkR

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.161 CTP-dependent riboflavin kinase

Cloned

Cloned on EC 2.7.1.161 - CTP-dependent riboflavin kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene ribK, genomic organization
gene ribK, genomic organization, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS
gene ribK, genomic organization, recombinant expression of N-terminally His6-Smt3-tagged enzyme in Escherichia coli strain BL21(DE3). The Smt3 polypeptide is a yeast SUMO orthologue which enhances protein solubility
recombinant expression of the MJ0056 gene in Escherichia coli
recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide in the Escherichia coli cell extract