2.5.1.84: all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific]
This is an abbreviated version!
For detailed information about all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific], go to the full flat file.
Reaction
+ 7 isopentenyl diphosphate = 7 diphosphate +
Synonyms
all-trans-nonaprenyl-diphosphate synthase [geranyl-diphosphate specific], EC 2.5.1.11, nonaprenyl diphosphate synthase, nonaprenylpyrophosphate synthetase, OsSPS1, OsSPS2, solanesyl diphosphate synthase, solanesyl-diphosphate synthase, SolPP synthase, SPP synthase, SPP-synthase, SPS, Sps2, ubiquinone:trans-prenyltransferase
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Metals Ions
Metals Ions on EC 2.5.1.84 - all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific]
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Co2+
-
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme
Mg2+
Mn2+
Mg2+
-
enzyme requires Mg2+, variable product specificity depending on Mg2+ concentration
Mg2+
-
Mg2+ is absolutely required for activity, Mn2+ is less effective. The chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and c40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds
Mg2+
-
the concentration of the complex between farnesyl diphosphate and Mg2+, the priming substrate for this synthase, does not affect the product distribution, provided that the concentration of isopentenyl diphosphate/Mg2+ is maintained at a certain level. The level of isopentenyl diphosphate/Mg2+ is decisive in affecting the chain length distribution of the products of the prenyltransferase reaction
Mg2+
-
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme
Mg2+
-
no activity in absence of divalent metal ion, activation with 2 mM Mg2+ is 50% of that obtained with Mn2+
Mn2+
-
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme
Mn2+
-
no activity in absence of divalent metal ion, 0.1 mM Mn2+ is required for optimal activity