Information on EC 2.5.1.84 - all-trans-nonaprenyl-diphosphate synthase [geranyl-diphosphate specific]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.84
-
RECOMMENDED NAME
GeneOntology No.
all-trans-nonaprenyl-diphosphate synthase [geranyl-diphosphate specific]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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nonaprenyl diphosphate biosynthesis I
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Terpenoid backbone biosynthesis
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isoprenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 7 isopentenyl units)
(2E,6E)-Farnesyl diphosphate and geranylgeranyl diphosphate are less effective as substrates than geranyl diphosphate. The enzyme is involved in the synthesis of the side chain of menaquinone-9 [1]. In Oryza sativa the enzyme SPS2 is involved in providing solanesyl diphosphate for plastoquinone-9 formation [3].
CAS REGISTRY NUMBER
COMMENTARY hide
83745-07-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
solanesyl diphosphate is a precursor for solanesol biosynthesis. In the solanesol biosynthetic pathway, isopentenyl diphosphate is first isomerised to form dimethylallyl diphosphate by isopentenyl diphosphate isomerase, Ipi, which then binds to isopentenyl diphosphates one by one in the head-tail mode; solanesyl diphosphate is a precursor for solanesol biosynthesis. In the solanesol biosynthetic pathway, isopentenyl diphosphate is first isomerised to form dimethylallyl diphosphate diphsphate by isopentenyl diphosphate isomerase, Ipi, which then binds to isopentenyl diphosphates one by one in the head-tail mode
physiological function
isoform SPS2 weakly complements the yeast coq1 disruptant, which does not form ubiquinone. Transformants produce ubiquinone 9 instead of the native yeast product ubiquinone 6. SPS2 is involved in providing solanesyl diphosphate for plastochinone-9 formation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
44% of the rate with geranyl diphosphate
products range from C 15 to C45
-
?
all-trans-farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
dimethylallyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + nonaprenyl diphosphate
show the reaction diagram
18.1% of the activity with geranyl diphosphate, OsSPS2
-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + ?
show the reaction diagram
18% of the rate with geranyl diphosphate
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-
?
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + nonaprenyl diphosphate
show the reaction diagram
farnesyl imidodiphosphate + isopentenyl diphosphate
?
show the reaction diagram
-
-
-
-
?
farnesyl methylenediphosphonate + isopentenyl diphosphate
?
show the reaction diagram
-
-
-
-
?
farnesyl phosphosulfate + isopentenyl diphosphate
?
show the reaction diagram
-
-
-
-
?
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + nonaprenyl diphosphate
show the reaction diagram
geranyl diphosphate is the preferred substrate, OsSPS2
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-
?
geranyl diphosphate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranyl imidodiphosphate + isopentenyl diphosphate
?
show the reaction diagram
-
-
-
-
?
geranyl methylenediphosphonate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranyl phosphosulfate + isopentenyl diphosphate
?
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate
5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate + 5 isopentenyl diphosphate
5 diphosphate + nonaprenyl diphosphate
show the reaction diagram
34.6% of the activity with geranyl diphosphate, OsSPS2
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
Q653T6, Q75HZ9
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-
-
?
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + nonaprenyl diphosphate
show the reaction diagram
Q75HZ9
OsSP2 is involved in the supply of all-trans-nonaprenyl diphosphate for plastoquinone-9 biosynthesis
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-
?
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-O-octyl-beta-D-glucopyranoside
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0.1%, 44% inhibition
Ca2+
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1 mM (in absence of Mn2+ or Mg2+), complete inhibition
deoxycholate
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0.01%, 70% inhibition
farnesyl methylenediphosphate
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above 0.005 mM
farnesyl phosphosulfate
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above 0.1 mM
Imidodiphosphate
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Methylenediphosphonate
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SDS
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0.005%, 78% inhibition
Triton X-100
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0.05%, 53% inhibition
Zn2+
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1 mM (in absence of Mn2+ or Mg2+), complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bacitracin
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0.03-0.06%, 2fold stimulation
bovine serum albumin
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stimulates
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Digitonin
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; 0.005%, 29fold activation
taurodeoxycholate
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0.005%, 1.3fold activation
Tween 80
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0.05-0.1%, 2fold stimulation
Tween-80
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0.005%, 1.4fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025 - 0.00076
all-trans-farnesyl diphosphate
0.001
all-trans-farnesyl methylenediphosphonate
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pH 7.4, 37C
0.0052
farnesyl diphosphate
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pH 7.4, 37C
0.0057
Farnesyl imidodiphosphate
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pH 7.4, 37C
0.0133
farnesyl phosphosulfate
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pH 7.4, 37C
0.00075 - 0.0043
geranyl diphosphate
0.0077
Geranyl imidodiphosphate
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pH 7.4, 37C
0.0054
Geranyl methylenediphosphonate
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pH 7.4, 37C
0.0909
Geranyl phosphosulfate
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pH 7.4, 37C
0.017 - 0.023
isopentenyl diphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000787
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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in Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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pH 7.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression; high expression level
Manually annotated by BRENDA team
high expression; high expression level
Manually annotated by BRENDA team
low expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
plastidic isozyme OsSPS2
Manually annotated by BRENDA team
mitchondrial isozyme OsSPS1
Manually annotated by BRENDA team
; isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
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2 * 34000, SDS-PAGE
74000 - 78000
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gel filtration
78000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 34000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for at least 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in yeast
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