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2.5.1.81: geranylfarnesyl diphosphate synthase

This is an abbreviated version!
For detailed information about geranylfarnesyl diphosphate synthase, go to the full flat file.

Word Map on EC 2.5.1.81

Reaction

geranylgeranyl diphosphate
+
isopentenyl diphosphate
=
(2E,6E,10E,14E)-geranylfarnesyl diphosphate
+
diphosphate

Synonyms

AtIDS1, AtIDS10, AtIDS6, AtIDS7, AtIDS9, C25 farnesylgeranyl diphosphate synthase, C25 FGPP synthase, farnesyl diphosphate synthase, farnesylgeranyl diphosphate synthase, FGPP synthase, Fgs, GFDP synthase, GFDP-forming enzyme, GFDPS, GFPS, IDS3, Lc-GFDPS, MM_0789

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.81 geranylfarnesyl diphosphate synthase

Cloned

Cloned on EC 2.5.1.81 - geranylfarnesyl diphosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a glutathione S-transferase fusion protein
expression of wild-type and mutant enzymes in Escherichia coli
gene AtIDS1, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, and recombinant enzyme expression in the loss-of-function AtIDS11 mutant ggpps11-4, which has an embryolethal phenotype due to the lack of GGDP. AtIDS1, a predominantly GFDP-producing enzyme that nonetheless forms a sizable amount of GGDP, complements the embryo-lethal phenotype of ggpps11-4. Transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana
gene AtIDS10, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana
gene AtIDS6, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana
gene AtIDS7, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana
gene AtIDS9, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21 and of N-terminally His6-tagged enzyme in Arabidopsis thaliana ecotype Col-0 under control of CaMV 35S promoter via Agrobacterium tumefaciens strain GV3101 transfection method, and recombinant enzyme expression in the loss-of-function AtIDS11 mutant ggpps11-4, which has an embryolethal phenotype due to the lack of GGDP. Transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana
gene Lc-GFDPS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression in Escherichia coli, and in Arabidopsis thaliana driven by a double CaMV 35S promoter
-