Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 20 | highly activating | Leucosceptrum canum |
Cloned (Comment) | Organism |
---|---|
gene Lc-GFDPS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression in Escherichia coli, and in Arabidopsis thaliana driven by a double CaMV 35S promoter | Leucosceptrum canum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00165 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
0.00304 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Leucosceptrum canum | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by Mg2+ and Mn2+ | Leucosceptrum canum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + isopentenyl diphosphate | Leucosceptrum canum | - |
(2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucosceptrum canum | - |
gene Lc-GFDPS | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glandular trichome | high expression level | Leucosceptrum canum | - |
leaf | - |
Leucosceptrum canum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Leucosceptrum canum | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GFDP synthase | - |
Leucosceptrum canum |
GFDPS | - |
Leucosceptrum canum |
IDS3 | - |
Leucosceptrum canum |
Lc-GFDPS | - |
Leucosceptrum canum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leucosceptrum canum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000517 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
0.0000082 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leucosceptrum canum |
Organism | Comment | Expression |
---|---|---|
Leucosceptrum canum | application of salicilic acid and methyl jasmonate induce GFDPS transcript and sesterterpenoid accumulation | up |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis suggest that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. Lc-GFDPS clusters in subcluster Ia, which is dominated by GGDPS sequences | Leucosceptrum canum |
metabolism | biosynthesis of the C25 precursor geranylfarnesyl diphosphate to leucosceptroid sesterterpenoids is catalyzed by enzyme GFDPS that uses substrates geranylgeranyl diphosphate and Iisopentenyl diphosphate derived from the plastidial MEP pathway, overview | Leucosceptrum canum |
physiological function | the geranylfarnesyl diphosphate synthase provides the precursor for sesterterpenoid (C25) formation in the glandular trichomes of the mint species Leucosceptrum canum, the C25 terpenoids might play a defensive role | Leucosceptrum canum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
0.003 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum |