2.5.1.65: O-phosphoserine sulfhydrylase
This is an abbreviated version!
For detailed information about O-phosphoserine sulfhydrylase, go to the full flat file.
Word Map on EC 2.5.1.65
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2.5.1.65
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oropharyngeal
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aeropyrum
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pernix
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o-acetyl-l-serine
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o-acetylserine
- 2.5.1.65
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oropharyngeal
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aeropyrum
- pernix
- o-acetyl-l-serine
- o-acetylserine
Reaction
Synonyms
APE1586, ApOPSS, CysK2, CysM, O-acetyl-L-serine sulfhydrylase, O-acetylserine sulfhydrylase, O-phospho-L-serine sulfhydrylase, O-phospho-L-serine-dependent S-sulfocysteine synthase, O-phosphoserine S-sulfocysteine synthase, O-phosphoserine specific cysteine synthase, O-phosphoserine(thiol)-lyase, OASS, OPSS, Rv1336, S-sulfocysteine synthase
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Purification
Purification on EC 2.5.1.65 - O-phosphoserine sulfhydrylase
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Ni-NTA column chromatography and Superdex-200 gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration, followed by cleavage of the His-tag using thrombin and tag elimination through another sequence of nickel affinity chromatography and gel filtration
recombinant wild-type, selenomethinonine-labeled, and mutant R297A enzymes from Escherichia coli
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