Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | slightly activates the O-phospho-L-serine sulfhydrylation reaction | Aeropyrum pernix |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta (DE3) | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-chloro-D-alanine | 18% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
3-Cyano-L-alanine | 42% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Cd2+ | slightly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
CdCl2 | 25°C, 10 min, 26% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 15% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Co2+ | strongly inhibits O-acetyl-L-serine sulfhydrylation, moderately inhibites O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
CoCl2 | 25°C, 10 min, 61% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 98.8% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Cu2+ | strongly inhibits O-acetyl-L-serine sulfhydrylation, moderately inhibites O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
CuCl2 | 25°C, 10 min, 79% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 98.7% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Fe2+ | slightly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
Fe3+ | strongly inhibits O-acetyl-L-serine sulfhydrylation, slightly inhibites O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
FeCl2 | 25°C, 10 min, 20% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 27% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
FeCl3 | 25°C, 10 min, 25% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 96.1% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Hg2+ | strongly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
HgCl2 | 25°C, 10 min, 98.3% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 80% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
additional information | no inhibition by O-phospho-D-serine, EDTA, 2-mercaptoethanol, DTT, NEM, PCMB, and Gd3+, while Ca2+, K+, Na+, Mn2+, and Mg2+ are poor inhibitors; no inhibition of the O-acetyl-L-serine sulfhydrylation reaction by O-phospho-D-serine | Aeropyrum pernix | |
Ni2+ | strongly inhibits O-acetyl-L-serine sulfhydrylation, slightly inhibites O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
NiCl2 | 25°C, 10 min, 15% inhibition of the O-phospho-L-serine sulfhydrylation reaction, almost complete inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
O-benzyl-L-serine | 36% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
O-tert-butyl-L-serine | 49% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Pb(CH3COO)2 | 25°C, 10 min, 95% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 88% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
Pb2+ | strongly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
Zn2+ | slightly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation | Aeropyrum pernix | |
ZnCl2 | 25°C, 10 min, 23% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 25% inhibition of the O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Aeropyrum pernix | |
additional information | - |
additional information | ping-pong bi-bi mechanism | Aeropyrum pernix | |
0.25 | - |
Sulfide | 60°C, O-acetyl-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
0.25 | - |
hydrogen sulfide | pH 7.6, 60°C, with O-acetyl-L-serine | Aeropyrum pernix | |
5 | - |
Sulfide | 60°C, O-phospho-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
5 | - |
hydrogen sulfide | pH 7.6, 60°C, with O-phospho-L-serine | Aeropyrum pernix | |
12.5 | - |
Sulfide | 85°C, O-phospho-L-serine sulfhydrylation reaction | Aeropyrum pernix | |
12.5 | - |
hydrogen sulfide | pH 7.6, 85°C, with O-phospho-L-serine | Aeropyrum pernix | |
21 | - |
O-acetyl-L-serine | 60°C | Aeropyrum pernix | |
21 | - |
O-acetyl-L-serine | pH 7.6, 60°C | Aeropyrum pernix | |
200 | - |
O-phospho-L-serine | 60°C | Aeropyrum pernix | |
200 | - |
O-phospho-L-serine | pH 7.6, 60°C | Aeropyrum pernix | |
250 | - |
O-phospho-L-serine | 85°C | Aeropyrum pernix | |
250 | - |
O-phospho-L-serine | pH 7.6, 85°C | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000 | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeropyrum pernix | L-cysteine biosynthesis | ? | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Aeropyrum pernix | enzyme is involved in L-cysteine biosynthesis, pathway overview | L-cysteine + acetate | - |
ir | |
O-phospho-L-serine + hydrogen sulfide | Aeropyrum pernix | enzyme is involved in L-cysteine biosynthesis, pathway overview | L-cysteine + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Aeropyrum pernix | - |
hyperthermophilic archeon | - |
Purification (Comment) | Organism |
---|---|
recombinant OASS | Aeropyrum pernix |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-phospho-L-serine + hydrogen sulfide = L-cysteine + phosphate | ping-pong bi-bi mechanism | Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.5 | - |
60°C, O-acetyl-L-serine sulfhydrylation reaction, recombinant OASS | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-L-alanine + hydrogen sulfide | - |
Aeropyrum pernix | ? | - |
ir | |
3-chloro-L-alanine + sulfide | heat-labile substrate, 173% of activity compared with O-acetyl-L-serine as substrate | Aeropyrum pernix | ? | - |
? | |
L-azaserine + hydrogen sulfide | O-phospho-L-serine is a heat-stable substrate | Aeropyrum pernix | ? | - |
ir | |
L-azaserine + sulfide | same activity as with O-acetyl-L-serine as substrate | Aeropyrum pernix | ? | - |
? | |
additional information | L-cysteine biosynthesis | Aeropyrum pernix | ? | - |
? | |
additional information | not: 3-chloro-D-alanine, 3-cyano-L-alanine, O-benzyl-L-serine, O-tert-butyl-L-serine, O-phospho-D-serine, O-succinyl-L-homoserine, L-homoserine | Aeropyrum pernix | ? | - |
? | |
additional information | substrate specificity, no activity with 3-chloro-D-alanine, 3-cyano-L-alanine, O-benzyl-L-serine, O-tert-butyl-L-serine, O-phospho-D-serine, O-succinyl-L-homoserine, and L-homoserine | Aeropyrum pernix | ? | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | enzyme is involved in L-cysteine biosynthesis, pathway overview | Aeropyrum pernix | L-cysteine + acetate | - |
ir | |
O-acetyl-L-serine + hydrogen sulfide | O-acetyl-L-serine is a heat-labile substrate | Aeropyrum pernix | L-cysteine + acetate | - |
ir | |
O-acetyl-L-serine + sulfide | heat-labile substrate | Aeropyrum pernix | L-cysteine + acetic acid | - |
? | |
O-phospho-L-serine + hydrogen sulfide | enzyme is involved in L-cysteine biosynthesis, pathway overview | Aeropyrum pernix | L-cysteine + phosphate | - |
ir | |
O-phospho-L-serine + hydrogen sulfide | O-phospho-L-serine is a heat-stable substrate | Aeropyrum pernix | L-cysteine + phosphate | - |
ir | |
O-phospho-L-serine + sulfide | heat-stabile substrate, 219% of activity compared with O-acetyl-L-serine as substrate, best substrate at pH 6.7 and 60°C, formation of an alpha-aminoacrylate intermediate between O-phospho-L-serine and pyridoxal 5-phosphate | Aeropyrum pernix | L-cysteine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000 | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine sulfhydrylase | - |
Aeropyrum pernix |
OASS | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
sulfhydrylation of 3-chloro-L-alanine | Aeropyrum pernix |
70 | - |
with substrate 3-chloro-L-alanine | Aeropyrum pernix |
80 | - |
sulfhydrylation of L-azaserine | Aeropyrum pernix |
80 | - |
with substrate L-azaserine | Aeropyrum pernix |
90 | - |
sulfhydrylation of O-phospho-L-serine | Aeropyrum pernix |
90 | - |
with substrate O-phospho-L-serine | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
50% of maximum activity with 3-chloro-L-alanine as substrate | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
156 | - |
O-acetyl-L-serine | 60°C | Aeropyrum pernix | |
156 | - |
O-acetyl-L-serine | pH 7.6, 60°C | Aeropyrum pernix | |
3050 | - |
O-phospho-L-serine | 60°C | Aeropyrum pernix | |
3050 | - |
O-phospho-L-serine | pH 7.6, 60°C | Aeropyrum pernix | |
14000 | - |
O-phospho-L-serine | 85°C | Aeropyrum pernix | |
14000 | - |
O-phospho-L-serine | pH 7.6, 85°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.1 | at 85°C | Aeropyrum pernix |
7.3 | 8.1 | dependent on the substrate, overview | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Aeropyrum pernix | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent | Aeropyrum pernix |