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0.007 - 0.66
2-deoxy-D-ribose 5-phosphate
0.12
2-deoxyribose 5-phosphate
-
pH 7.6, 37°C
0.01 - 9
D-arabinose 5-phosphate
1.1
L-xylose 5-phosphate
at 30°C
0.02 - 100
phosphoenolpyruvate
additional information
additional information
-
0.007
2-deoxy-D-ribose 5-phosphate
-
mutant N59A, pH 7.5, temperature not specified in the publication
0.13
2-deoxy-D-ribose 5-phosphate
-
wild-type, pH 7.5, temperature not specified in the publication
0.202
2-deoxy-D-ribose 5-phosphate
-
mutant N57A, pH 7.5, temperature not specified in the publication
0.66
2-deoxy-D-ribose 5-phosphate
-
wild-type, pH 7.5, temperature not specified in the publication
0.01
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, R117A
0.108
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, mutant F114R/R117A
0.14
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, F114R/R117Q/F139G
0.149
D-arabinose 5-phosphate
-
mutant N59A, pH 7.5, temperature not specified in the publication
0.178
D-arabinose 5-phosphate
-
mutant N59DELTA, pH 7.5, temperature not specified in the publication
0.27
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, mutant F114R/R117Q
0.3
D-arabinose 5-phosphate
-
mutant N57A, pH 7.5, temperature not specified in the publication
0.32
D-arabinose 5-phosphate
mutant enzyme R1096G
0.333
D-arabinose 5-phosphate
-
0.36
D-arabinose 5-phosphate
pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated
0.38
D-arabinose 5-phosphate
-
pH 7.5, 60°C
0.42
D-arabinose 5-phosphate
pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated
0.48
D-arabinose 5-phosphate
wild-type enzyme
0.49
D-arabinose 5-phosphate
-
pH 6.5, 25°C, Zn2+-enzyme
0.74
D-arabinose 5-phosphate
-
pH 7.4, 25°C, mutant enzyme P145S
0.87
D-arabinose 5-phosphate
-
pH 7.5, 70°C
1
D-arabinose 5-phosphate
-
pH 7.4, 37°C
1.06
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, R117Q
1.08
D-arabinose 5-phosphate
-
pH 6.5, 25°C, Cd2+-enzyme
1.47
D-arabinose 5-phosphate
-
pH 7.5, 80°C
1.63
D-arabinose 5-phosphate
-
pH 7.4, 37°C, mutant enzyme P145S
1.75
D-arabinose 5-phosphate
-
pH 7.4, 25°C, wild-type enzyme
1.9
D-arabinose 5-phosphate
pH 7.5, 37°C, Cd2+ mutant enzyme N26C
1.9
D-arabinose 5-phosphate
pH 7.5, 37°C, Mn2+ mutant enzyme N26C
2
D-arabinose 5-phosphate
-
pH 7.5, 90°C
2.7
D-arabinose 5-phosphate
at 30°C
3
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, mutant F114R
4
D-arabinose 5-phosphate
-
60°C, pH 7.0, 0.48 mM Cd2+
4.4
D-arabinose 5-phosphate
-
mutant P245A, pH 7.2, 37°C
4.8
D-arabinose 5-phosphate
-
wild-type, pH 7.2, 37°C
4.8
D-arabinose 5-phosphate
-
wild-type, pH 7.5, temperature not specified in the publication
4.8
D-arabinose 5-phosphate
-
mutant D243E, pH 7.2, 37°C
4.8
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, R117K
5.31
D-arabinose 5-phosphate
-
pH 6.5, 25°C
5.6
D-arabinose 5-phosphate
-
pH 7.4, 37°C, wild-type enzyme
5.9
D-arabinose 5-phosphate
-
37°C
5.9
D-arabinose 5-phosphate
pH and temperature not specified in the publication, recombinant enzyme
6.1
D-arabinose 5-phosphate
pH 7.5, 37°C, wild-type enzyme
6.1
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, mutant F114A
6.8
D-arabinose 5-phosphate
-
pH 7.6, 37°C
6.8
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication
8
D-arabinose 5-phosphate
-
wild-type, pH 7.5, temperature not specified in the publication
8
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, wild/type enzyme
8.6
D-arabinose 5-phosphate
-
pH and temperature not specified in the publication, mutant F139A
9
D-arabinose 5-phosphate
pH 7.5, 60°C, wild-type Mn2+-enzyme
0.02
phosphoenolpyruvate
N23C/C246S mutant control, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
0.095
phosphoenolpyruvate
-
0.1
phosphoenolpyruvate
-
pH 7.8, Co2+-reconstituted enzyme
0.1
phosphoenolpyruvate
-
pH 7.8, Cu2+-reconstituted enzyme
0.3
phosphoenolpyruvate
-
pH 7.8, Zn2+-reconstituted enzyme
0.31
phosphoenolpyruvate
N23C/C247E/P249A mutant control, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
0.31
phosphoenolpyruvate
recombinant enzyme produced in Escherichia coli, native Zn2+ and Fe2+ not substituted, 100 mM Tris-acetate, pH 7.5, 40°C
0.32
phosphoenolpyruvate
mutant enzyme R106G
0.333
phosphoenolpyruvate
-
0.34
phosphoenolpyruvate
enzyme preparation 1, substitution with 5 microM Cu2+, 100 mM Tris-acetate, pH 7.5, 40°C
0.36
phosphoenolpyruvate
pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated
0.36
phosphoenolpyruvate
enzyme preparation 1, substitution with 5 microM Cu2+, pH 5.0, 40°C
0.38
phosphoenolpyruvate
-
pH 7.5, 60°C
0.38
phosphoenolpyruvate
N23C/C247E mutant control, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
0.38
phosphoenolpyruvate
N23C/P249A mutant control, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
0.41
phosphoenolpyruvate
enzyme preparation 1, substitution with 50 microM Cd2+, 100 mM Tris-acetate, pH 7.5, 40°C
0.42
phosphoenolpyruvate
pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated
0.46
phosphoenolpyruvate
independent enzyme preparation 2, substitution with 50 microM Cd2+, pH 5.0, 40°C
0.47
phosphoenolpyruvate
enzyme preparation 1, substitution with 50 microM Zn2+, 100 mM Tris-acetate, pH 7.5, 40°C
0.48
phosphoenolpyruvate
wild-type enzyme
0.48
phosphoenolpyruvate
independent preparation 2 enzyme, substitution with 50 microM Cd2+, 100 mM Tris-acetate, pH 7.5, 40°C
0.49
phosphoenolpyruvate
-
pH 6.5, 25°C, Zn2+-enzyme
0.72
phosphoenolpyruvate
-
pH 7.4, 25°C, mutant enzyme P145S
0.87
phosphoenolpyruvate
-
pH 7.5, 70°C
0.93
phosphoenolpyruvate
N23C mutant control, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
0.94
phosphoenolpyruvate
N23C/P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
1
phosphoenolpyruvate
-
pH 7.4, 37°C
1
phosphoenolpyruvate
-
pH 7.8, Cd2+-reconstituted enzyme
1.08
phosphoenolpyruvate
-
pH 6.5, 25°C, Cd2+-enzyme
1.42
phosphoenolpyruvate
N23C mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
1.47
phosphoenolpyruvate
-
pH 7.5, 80°C
1.9
phosphoenolpyruvate
pH 7.5, 37°C, Cd2+ mutant enzyme N26C
1.9
phosphoenolpyruvate
pH 7.5, 37°C, Mn2+ mutant enzyme N26C
2
phosphoenolpyruvate
-
pH 7.5, 90°C
2.02
phosphoenolpyruvate
-
pH 7.4, 37°C, mutant enzyme P145S
2.02
phosphoenolpyruvate
N23C/C247E/P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
2.08
phosphoenolpyruvate
-
pH 7.4, 25°C, wild-type enzyme
2.71
phosphoenolpyruvate
N23C/C246S mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
3.27
phosphoenolpyruvate
N23C/C247E mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
3.6
phosphoenolpyruvate
D247E/P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
3.9
phosphoenolpyruvate
N23C/C246S/D247E mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
4
phosphoenolpyruvate
-
60°C, pH 7.0, 0.48 mM Cd2+
4.4
phosphoenolpyruvate
D247E mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
4.4
phosphoenolpyruvate
N23C/C246S/D247E/P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
4.79
phosphoenolpyruvate
N23C/C246S/P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 90 microM MnSO4.H2O
5.31
phosphoenolpyruvate
-
pH 6.5, 25°C
5.5
phosphoenolpyruvate
C246S mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
5.9
phosphoenolpyruvate
-
37°C
5.9
phosphoenolpyruvate
pH and temperature not specified in the publication, recombinant enzyme
6.1
phosphoenolpyruvate
pH 7.5, 37°C, wild-type enzyme
6.7
phosphoenolpyruvate
-
pH 7.4, 37°C, wild-type enzyme
6.8
phosphoenolpyruvate
-
pH and temperature not specified in the publication
8
phosphoenolpyruvate
wild type, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
8.6
phosphoenolpyruvate
P249A mutant, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
9
phosphoenolpyruvate
pH 7.5, 60°C, wild-type Mn2+-enzyme
100
phosphoenolpyruvate
-
in 10 mM ammonium acetate buffer, pH 7.8
additional information
additional information
N23C/C246S/D247E mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-
additional information
additional information
-
N23C/C246S/D247E mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-
additional information
additional information
N23C/C246S/D247E/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-
additional information
additional information
-
N23C/C246S/D247E/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-
additional information
additional information
N23C/C246S/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-
additional information
additional information
-
N23C/C246S/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA
-