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Literature summary for 2.5.1.55 extracted from
- Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase (2008), Bioorg. Med. Chem., 16, 9830-9836.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Neisseria meningitidis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N59A | mutant retains less than 1% of the wild type activity | Neisseria meningitidis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
phosphoenolpyruvate | Km less than 0.001 mM, in the presence of D-arabinose 5-phosphate, at 30°C | Neisseria meningitidis |  |
| 0.0025 | - |
phosphoenolpyruvate | in the presence of L-xylose 5-phosphate, at 30°C | Neisseria meningitidis | |
| 0.012 | - |
D-arabinose 5-phosphate | at 30°C | Neisseria meningitidis | |
| 0.057 | - |
L-xylose 5-phosphate | at 30°C | Neisseria meningitidis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Neisseria meningitidis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria meningitidis | Q9JZ55 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Source 15Q column chromatography and Source 15Phe column chromatography | Neisseria meningitidis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
crude cell lysate, at 25°C and pH 7.5 | Neisseria meningitidis |
| 2 | - |
purified enzyme, at 25°C and pH 7.5 | Neisseria meningitidis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | D-ribose 5-phosphate, D-lyxose 5-phosphate, and D-xylose 5-phosphate with altered configuration at C2, C3 or both C2 and C3 relative to D-arabinose 5-phosphate are no substrates for KDO8P synthase | Neisseria meningitidis | ? | - |
? | |
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Neisseria meningitidis | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + L-xylose 5-phosphate + H2O | - |
Neisseria meningitidis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase | - |
Neisseria meningitidis |
| Kdo8P synthase | - |
Neisseria meningitidis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
L-xylose 5-phosphate | at 30°C | Neisseria meningitidis | |
| 2.7 | - |
D-arabinose 5-phosphate | at 30°C | Neisseria meningitidis | |
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Release 2023.1 (January 2023)
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