Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
N59A | mutant retains less than 1% of the wild type activity | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
phosphoenolpyruvate | Km less than 0.001 mM, in the presence of D-arabinose 5-phosphate, at 30°C | Neisseria meningitidis | |
0.0025 | - |
phosphoenolpyruvate | in the presence of L-xylose 5-phosphate, at 30°C | Neisseria meningitidis | |
0.012 | - |
D-arabinose 5-phosphate | at 30°C | Neisseria meningitidis | |
0.057 | - |
L-xylose 5-phosphate | at 30°C | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | Q9JZ55 | - |
- |
Purification (Comment) | Organism |
---|---|
Source 15Q column chromatography and Source 15Phe column chromatography | Neisseria meningitidis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
crude cell lysate, at 25°C and pH 7.5 | Neisseria meningitidis |
2 | - |
purified enzyme, at 25°C and pH 7.5 | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | D-ribose 5-phosphate, D-lyxose 5-phosphate, and D-xylose 5-phosphate with altered configuration at C2, C3 or both C2 and C3 relative to D-arabinose 5-phosphate are no substrates for KDO8P synthase | Neisseria meningitidis | ? | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Neisseria meningitidis | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + L-xylose 5-phosphate + H2O | - |
Neisseria meningitidis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-octulosonate 8-phosphate synthase | - |
Neisseria meningitidis |
Kdo8P synthase | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
L-xylose 5-phosphate | at 30°C | Neisseria meningitidis | |
2.7 | - |
D-arabinose 5-phosphate | at 30°C | Neisseria meningitidis |