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Literature summary for 2.5.1.55 extracted from

  • Allison, T.M.; Yeoman, J.A.; Hutton, R.D.; Cochrane, F.C.; Jameson, G.B.; Parker, E.J.
    Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans (2010), Biochim. Biophys. Acta, 1804, 1526-1536.
    View publication on PubMed
Search for more literature for 2.5.1.55

Protein Variants

Protein Variants Comment Organism
C21N complete loss of activity Acidithiobacillus ferrooxidans
D243A mutation of the absolutely conserved 243AspGlyPro245 motif, complete loss of activity Acidithiobacillus ferrooxidans
D243Q mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency Acidithiobacillus ferrooxidans
P245A mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 phosphoenolpyruvate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
0.012
-
 phosphoenolpyruvate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
0.013
-
 phosphoenolpyruvate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
0.021
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
0.024
-
 D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
2.63
-
 D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ presence of Cd2+ restores activity to metal-free enzyme, Cd2+ significantly enhances the stability of the enzyme and raises the Tm by 14°C Acidithiobacillus ferrooxidans
Co2+ presence of Co2+ restores activity to metal-free enzyme Acidithiobacillus ferrooxidans
Mn2+ presence of Mn2+ restores activity to metal-free enzyme Acidithiobacillus ferrooxidans
additional information divalent metal ion required, with Mn2+, Co2+ and Cd2+ in decreasing order being able to restore activity to metal-free enzyme Acidithiobacillus ferrooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information D-glucose 6-phosphate and D-erythrose 4-phosphate are no substrates Acidithiobacillus ferrooxidans ?
-
 ?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate 2-deoxy-D-ribose 5-phosphate is a poor substrate Acidithiobacillus ferrooxidans ? + phosphate
-
 ?
phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Acidithiobacillus ferrooxidans 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
 wild-type, melting temperature Acidithiobacillus ferrooxidans
68
-
 mutant C21N, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
68
-
 mutant D243E, melting temperature Acidithiobacillus ferrooxidans
69
-
 mutant C21N, melting temperature Acidithiobacillus ferrooxidans
71
-
 mutant P245A, melting temperature Acidithiobacillus ferrooxidans
78
-
 mutant D243E, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
79
-
 mutant P245A, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans
82
-
 wild-type, presence of Cd2+, melting temperature Acidithiobacillus ferrooxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
4.8
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
4.8
-
 D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 D-arabinose 5-phosphate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
180
-
 D-arabinose 5-phosphate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans
230
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
370
-
 phosphoenolpyruvate mutant D243E, pH 7.2, 37°C Acidithiobacillus ferrooxidans
400
-
 phosphoenolpyruvate wild-type, pH 7.2, 37°C Acidithiobacillus ferrooxidans
850
-
 phosphoenolpyruvate mutant P245A, pH 7.2, 37°C Acidithiobacillus ferrooxidans