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2.5.1.3: thiamine phosphate synthase

This is an abbreviated version!
For detailed information about thiamine phosphate synthase, go to the full flat file.

Reaction

4-amino-2-methyl-5-(diphosphomethyl)pyrimidine
+
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
=
diphosphate
 +
thiamine phosphate
 +
CO2

Synonyms

2-methyl-4-amino-5-hydroxymethylpyrimidinepyrophosphate:4-methyl-5-(2'-phosphoethyl)-thiazole 2-methyl-4-aminopyrimidine-5-methenyltransferase, hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase, PF0544, pyrophosphorylase, thiamin phosphate, SSO2532, TH16, THI6, thiamin monophosphate pyrophosphorylase, thiamin phosphate diphosphorylase, thiamin phosphate pyrophosphorylase, thiamin phosphate synthase, thiamin-phosphate pyrophosphorylase, thiamine monophosphate pyrophosphorylase, thiamine phosphate pyrophosphorylase, thiamine phosphate synthase, thiamine-phosphate diphosphorylase, thiamine-phosphate pyrophosphorylase, thiamine-phosphate synthase, thiaminephosphate pyrophosphorylase, ThiE, ThiL, ThiN protein, TM0723, TM1872, TMP pyrophosphorylase, TMP-PPase, TMPPase, TMPS, TPS, YjbQ

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.3 thiamine phosphate synthase

Engineering

Engineering on EC 2.5.1.3 - thiamine phosphate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R320M
activity is 1% compared to wild-type enzyme
H341L
-
no activity
-
R320M
-
activity is 1% compared to wild-type enzyme
-
M134K
phosphomethylpyrimidine kinase activity similar to wild-type, thiamin-phosphate diphosphorylase activity almost completely abolished
M134K/T472D
83-97% residual phosphomethylpyrimidine kinase activity, 19-32% residual thiamin-phosphate diphosphorylase activity
Q373L
phosphomethylpyrimidine kinase activity similar to wild-type, 64-75% residual thiamin-phosphate diphosphorylase activity
Q98L
phosphomethylpyrimidine kinase activity similar to wild-type, thiamin-phosphate diphosphorylase activity almost completely abolished
Q98L/Q373L
phosphomethylpyrimidine kinase activity similar to wild-type, 7-13% residual thiamin-phosphate diphosphorylase activity
S444A
almost complete loss of phosphomethylpyrimidine kinase activity, 80-91% residual thiamin-phosphate diphosphorylase activity
T472D
phosphomethylpyrimidine kinase activity similar to wild-type, 46-54% residual thiamin-phosphate diphosphorylase activity
additional information
-
in enzyme deletion mutant, no growth difference is observed when compared to the wild-type strain