Literature summary for 2.5.1.3 extracted from

Morett, E.; Saab-Rincon, G.; Olvera, L.; Olvera, M.; Flores, H.; Grande, R.
Sensitive genome-wide screen for low secondary enzymatic activities: the YjbQ family shows thiamin phosphate synthase activity (2008), J. Mol. Biol., 376, 839-853.

Search for more literature for 2.5.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli
overexpression of YjbQ complements the thiamin auxotrophy of the Escherichia coli MC1061DELTAthiE strain, in spite of their thiamine phosphate synthase activity being several orders of magnitude lower than that of the thiE-coded enzyme	Pyrococcus furiosus
overexpression of YjbQ complements the thiamin auxotrophy of the Escherichia coli MC1061DELTAthiE strain, in spite of their thiamine phosphate synthase activity being several orders of magnitude lower than that of the thiE-coded enzyme	Thermotoga maritima
overexpression of YjbQ complements the thiamin auxotrophy of the Escherichia coli MC1061DELTAthiE strain, in spite of their thiamine phosphate synthase activity being several orders of magnitude lower than that of the thiE-coded enzyme	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
additional information	in enzyme deletion mutant, no growth difference is observed when compared to the wild-type strain	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Pyrococcus furiosus	Q8U3C6	-	-
Saccharolobus solfataricus	Q97VS8	-	-
Saccharolobus solfataricus P2	Q97VS8	-	-
Thermotoga maritima	Q9WZI2	-	-
Thermotoga maritima	Q9X2I5	-	-
Thermotoga maritima DSM 3109	Q9WZI2	-	-
Thermotoga maritima DSM 3109	Q9X2I5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole	very low activity, almost 400-fold lower than the activity shown by the thiE-encoded thiamine-phosphate diphosphatase of Escherichia coli	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
PF0544	locus name	Pyrococcus furiosus
SSO2532	locus name	Saccharolobus solfataricus
thiamin phosphate synthase	-	Pyrococcus furiosus
thiamin phosphate synthase	-	Thermotoga maritima
thiamin phosphate synthase	-	Saccharolobus solfataricus
TM0723	locus name	Thermotoga maritima
TM1872	locus name	Thermotoga maritima
YjbQ	-	Pyrococcus furiosus
YjbQ	-	Thermotoga maritima
YjbQ	-	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)