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2.5.1.3: thiamine phosphate synthase

This is an abbreviated version!
For detailed information about thiamine phosphate synthase, go to the full flat file.

Reaction

4-amino-2-methyl-5-(diphosphomethyl)pyrimidine
+
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
=
diphosphate
+
thiamine phosphate
+
CO2

Synonyms

2-methyl-4-amino-5-hydroxymethylpyrimidinepyrophosphate:4-methyl-5-(2'-phosphoethyl)-thiazole 2-methyl-4-aminopyrimidine-5-methenyltransferase, hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase, PF0544, pyrophosphorylase, thiamin phosphate, SSO2532, TH16, THI6, thiamin monophosphate pyrophosphorylase, thiamin phosphate diphosphorylase, thiamin phosphate pyrophosphorylase, thiamin phosphate synthase, thiamin-phosphate pyrophosphorylase, thiamine monophosphate pyrophosphorylase, thiamine phosphate pyrophosphorylase, thiamine phosphate synthase, thiamine-phosphate diphosphorylase, thiamine-phosphate pyrophosphorylase, thiamine-phosphate synthase, thiaminephosphate pyrophosphorylase, ThiE, ThiL, ThiN protein, TM0723, TM1872, TMP pyrophosphorylase, TMP-PPase, TMPPase, TMPS, TPS, YjbQ

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.3 thiamine phosphate synthase

Reference

Reference on EC 2.5.1.3 - thiamine phosphate synthase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Camiener, G.W.; Brown, G.M.
The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates
J. Biol. Chem.
235
2411-2417
1960
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kawasaki, Y.
Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway
J. Bacteriol.
175
5153-5158
1993
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Leder, I.G.
The enzymatic synthesis of thiamine monophosphate
J. Biol. Chem.
236
3066-3071
1961
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kayama, Y.; Kawasaki, T.
Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli
Arch. Biochem. Biophys.
158
242-248
1973
Escherichia coli
Manually annotated by BRENDA team
Kawasaki, T.
Thiamine phosphate pyrophosphorylase
Methods Enzymol.
62
69-73
1979
Escherichia coli
Manually annotated by BRENDA team
Reddick, J.J.; Nicewonger, R.; Begley, T.P.
Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism
Biochemistry
40
10095-10102
2001
Bacillus subtilis
Manually annotated by BRENDA team
Nosaka, K.; Nishimura, H.; Kawasaki, Y.; Tsujihara, T.; Iwashima, A.
Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae
J. Biol. Chem.
269
30510-30516
1994
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kim, Y.S.; Nosaka, K.; Downs, D.M.; Kwak, J.M.; Park, D.; Chung, I.K.; Nam, H.G.
A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis
Plant Mol. Biol.
37
955-966
1998
Brassica napus
Manually annotated by BRENDA team
Chiu, H.J.; Reddick, J.J.; Begley, T.P.; Ealick, S.E.
Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution
Biochemistry
38
6460-6470
1999
Bacillus subtilis (P39594), Bacillus subtilis
Manually annotated by BRENDA team
Golda, A.; Szyniarowski, P.; Ostrowska, K.; Kozik, A.; Rapala-Kozik, M.
Thiamine binding and metabolism in germinating seeds of selected cereals and legumes
Plant Physiol. Biochem.
42
187-195
2004
Avena sativa, Vicia faba, Pisum sativum, Zea mays
Manually annotated by BRENDA team
Ajjawi, I.; Tsegaye, Y.; Shintani, D.
Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1
Arch. Biochem. Biophys.
459
107-114
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Rapala-Kozik, M.; Olczak, M.; Ostrowska, K.; Starosta, A.; Kozik, A.
Molecular characterization of thi3 gene involved in thiamine biosynthesis in Zea mays: cDNA sequence and enzymatic and structural properties of recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamin
Biochem. J.
408
149-159
2007
Zea mays (Q2UVH9)
Manually annotated by BRENDA team
Karunakaran, R.; Ebert, K.; Harvey, S.; Leonard, M.E.; Ramachandran, V.; Poole, P.S.
Thiamine is synthesized by a salvage pathway in Rhizobium leguminosarum bv. viciae strain 3841
J. Bacteriol.
188
6661-6668
2006
Escherichia coli
Manually annotated by BRENDA team
Morett, E.; Saab-Rincon, G.; Olvera, L.; Olvera, M.; Flores, H.; Grande, R.
Sensitive genome-wide screen for low secondary enzymatic activities: the YjbQ family shows thiamin phosphate synthase activity
J. Mol. Biol.
376
839-853
2008
Escherichia coli, Pyrococcus furiosus (Q8U3C6), Saccharolobus solfataricus (Q97VS8), Saccharolobus solfataricus P2 (Q97VS8), Thermotoga maritima (Q9WZI2), Thermotoga maritima (Q9X2I5), Thermotoga maritima DSM 3109 (Q9WZI2), Thermotoga maritima DSM 3109 (Q9X2I5)
Manually annotated by BRENDA team
Kowalska, E.; Kozik, A.
The genes and enzymes involved in the biosynthesis of thiamin and thiamin diphosphate in yeasts
Cell. Mol. Biol. Lett.
13
271-282
2008
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Hazra, A.B.; Han, Y.; Chatterjee, A.; Zhang, Y.; Lai, R.Y.; Ealick, S.E.; Begley, T.P.
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase
J. Am. Chem. Soc.
133
9311-9319
2011
Bacillus subtilis (P39594), Bacillus subtilis 168 (P39594)
Manually annotated by BRENDA team
Paul, D.; Chatterjee, A.; Begley, T.P.; Ealick, S.E.
Domain organization in Candida glabrata THI6, a bifunctional enzyme required for thiamin biosynthesis in eukaryotes
Biochemistry
49
9922-9934
2010
[Candida] glabrata
Manually annotated by BRENDA team
Saab-Rincon, G.; Olvera, L.; Olvera, M.; Rudino-Pinera, E.; Benites, E.; Soberon, X.; Morett, E.
Evolutionary walk between (beta/alpha)8 barrels: catalytic migration from triosephosphate isomerase to thiamin phosphate synthase
J. Mol. Biol.
416
255-270
2012
Escherichia coli
Manually annotated by BRENDA team
Khare, G.; Kar, R.; Tyagi, A.K.
Identification of inhibitors against Mycobacterium tuberculosis thiamin phosphate synthase, an important target for the development of anti-TB drugs
PLoS ONE
6
e22441
2011
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Hayashi, M.; Kobayashi, K.; Esaki, H.; Konno, H.; Akaji, K.; Tazuya, K.; Yamada, K.; Nakabayashi, T.; Nosaka, K.
Enzymatic and structural characterization of an archaeal thiamin phosphate synthase
Biochim. Biophys. Acta
1844
803-809
2014
Pyrobaculum calidifontis (A3MSR0), Pyrobaculum calidifontis, Pyrobaculum calidifontis JCM 11548 (A3MSR0)
Manually annotated by BRENDA team
Tian, K.; Niu, D.; Liu, X.; Prior, B.A.; Zhou, L.; Lu, F.; Singh, S.; Wang, Z.
Limitation of thiamine pyrophosphate supply to growing Escherichia coli switches metabolism to efficient D-lactate formation
Biotechnol. Bioeng.
113
182-188
2016
Escherichia coli, Escherichia coli B0013
Manually annotated by BRENDA team