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2.4.1.182: lipid-A-disaccharide synthase

This is an abbreviated version!
For detailed information about lipid-A-disaccharide synthase, go to the full flat file.

Word Map on EC 2.4.1.182

Reaction

a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine
+
a lipid X
=
UDP
 +
a lipid A disaccharide

Synonyms

lcsC, lcsC/lpxB1, Legionella cytotoxic suppressor C, lipid A disaccharide synthase, lipid A disaccharide synthetase, LpxB, More, pgsB, synthase, lipid A disaccharide

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.182 lipid-A-disaccharide synthase

Cloned

Cloned on EC 2.4.1.182 - lipid-A-disaccharide synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli MN7 (temperature sensitive lpxB mutant), expression of protein complements growth at 30 and 42°C
expressed in Escherichia coli strain XL1-Blue
-
expression from plasmid, DNA sequence analysis, lpxB gene is cotranscribed with the lpxA gene located in the same operon
-
gene lpxB, recombinant expression of His6-tagged wild-type and mutant enzymes, coexpression of His6-tagged mutants LpxBR201A, LpxBN316A, and LpxBFN, and pull-down assays
gene lpxB, recombinant overexpression of LpxB in Escherichia coli resulting in the accumulation of aberrant tubular membranes of a uniform diameter along the inner surface of the bacterial inner membrane, suggesting that the accumulation of the lipid product of the LpxB reaction, 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine-1-phosphate (DSMP), is toxic to cells
overexpression from plasmid as His-tagged protein
-
overexpression in Escherichia coli from plasmid, alone and together with gene lpxA, UDP-GlcNAc acyltransferase
-