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2.4.1.182: lipid-A-disaccharide synthase

This is an abbreviated version!
For detailed information about lipid-A-disaccharide synthase, go to the full flat file.

Word Map on EC 2.4.1.182

Reaction

a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine
+
a lipid X
=
UDP
 +
a lipid A disaccharide

Synonyms

lcsC, lcsC/lpxB1, Legionella cytotoxic suppressor C, lipid A disaccharide synthase, lipid A disaccharide synthetase, LpxB, More, pgsB, synthase, lipid A disaccharide

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.182 lipid-A-disaccharide synthase

Engineering

Engineering on EC 2.4.1.182 - lipid-A-disaccharide synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D89A
-
mutant shows no residual catalytic activity
F298E/N316A
site-directed mutagenesis, mutant LpxBFN, the mutant shows 0.0078% of wild-type activity
L69S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
L72S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
L72S/L75S
site-directed mutagenesis, the mutant shows 1.74% of wild-type enzyme activity
L72S/L75S/L76S
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type enzyme
L72S/L76S
site-directed mutagenesis, the mutant shows 32.2% of wild-type enzyme activity
L75S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
L75S/L76S
site-directed mutagenesis, the mutant shows 30.6% of wild-type enzyme activity
L76S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
M207S
site-directed mutagenesis, structure determination as selenomethionine-labeled enzyme
N316A
site-directed mutagenesis, mutant LpxBFN, the mutant shows 0.34% of wild-type activity
R201A
V66S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
V66S/V68S/L69S
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type enzyme
V66S/V68S/L69S/L72S/L75S/L76S
site-directed mutagenesis, mutant LpxB6S , the mutation improves solubility and yield of LpxB and shows the least aggregation of all mutants on a size exclusion column
V68S
site-directed mutagenesis, the mutation improves solubility and yield of LpxB
D89A
-
mutant shows no residual catalytic activity
-
R201A
-
mutant shows no residual catalytic activity
-
additional information