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2.4.1.144: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.144

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
=
UDP
 +
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Synonyms

(GnT)-III, acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta4-, III, beta(1,4)-N-acetylglucosaminyltransferase III, beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, beta-1,4-N-acetylglucosaminyltransferase III, beta-D-mannoside beta-1,4-N-acetylglucosaminyltransferase, beta1,4-N-acetylglucosaminyltransferase III, EC 2.4.1.51, GlcNAc-transferase-III, GlcNAcTase-III, GnT-III, GnTIII, Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase III, MGAT3, N-acetyl-glucosaminyltransferase III, N-acetylglucosaminyltransferase III, N-acetylglucosaminyltransferase-III, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III, uridine diphosphate (UDP)-N-acetylglucosamin/beta-D-mannoside beta-1,4-N-acetylglucosaminyltransferase III, uridine diphosphoacetylglucosamine-glycopeptide beta4-acetylglucosaminyltransferase III

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.144 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Expression

Expression on EC 2.4.1.144 - beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
downregulation of GnT-III expression in MDA-MB-231 cells, an E-cadherin-deficient cell line, the MDCK cell, in which GnTIII expression is undetectable, and fibroblasts, which lack E-cadherin
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during epithelial-mesenchymal-transition, enzyme expression is dramatically decreased and later recoveres when cells return to an epithelial-like phenotype
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E-cadherin-mediated cell-cell interaction upregulates GnT-III expression, suggesting that regulation of GnT-III and E-cadherin expression may exist as a positive feedback loop
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E-cadherin-mediated cell-cell interaction upregulates GnT-III expression. Significant upregulation of GnT-III expression in epithelial cells that express basal levels of E-cadherin and GnT-III
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GnT-III knockdown in MCF-7/AZ cells through siRNA
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in MDA-MB231 cells, an E-cadherin-deficient cell line, GnT-III expression is undetectable
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increase of enzyme expression by wild-type E-cadherin
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knockdown of GnT-III, de-localization of E-cadherin and cytoplasmic accumulation, modification of N-glycosylation in E-cadherin
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shRNA knockdown of beta-catenin results in a dramatic increase in enzyme expression
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significant up-regulation of GnT-III expression in epithelial cells that express basal levels of E-cadherin and GnT-III. Expression levels of GnT-III and its products, the bisected N-glycans, are up-regulated by cell-cell interaction via the E-cadherin-catenin-actin complex. GnT-III expression is significantly up-regulated in cells cultured under dense conditions. Modest increase in GnT-III expression under dense culture conditions in alpha-catenin-deficient DLD-1 cells as well as in E-cadherin-deficient MDA-MB-231 cells. Mutual regulation of GnT-III expression and E-cadherin-mediated cell-cell interaction exists as a positive-feedback loop
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stimulation of the Wnt signaling pathway by the addition of exogenous Wnt3a or SB415286 consistently and significantly inhibits enzyme expression
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the expression levels of GnT-III are suppressed about 25fold in transforming growth factor-beta-treated cells (5 ng/ml)
wild-type E-cadherin regulates MGAT3 gene transcription resulting in increased GnT-III expression. GnT-III mRNA transcription levels in MDA-MB-435 cells transfected with E-cadherin wild-type are ca. 2fold higher than those in MDA-MB-435 mock cells
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