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2.4.1.131: GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.131

Reaction

2 GDP-alpha-D-mannose +

alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
= 2 GDP +
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Synonyms

Alg11, Alg2 mannosyltransferase, At2G40190, galactomannan deficiency protein 3, GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase, gmd3, guanosine diphosphomannose-oligosaccharide-lipid mannosyltransferase, hALG11, LEW3, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid, oligosaccharide-lipid mannosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.131 GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase

Engineering

Engineering on EC 2.4.1.131 - GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L85A
no effect on Alg11 mannosyltransferase activity
L86P
mutant enzyme does not complement the growth deffect of the mutant strain DELTAalgg3 (Saccharomyces cerevisiae)
L86S
mutant enzyme slightly complements the growth deffect of the mutant strain DELTAalgg3 (Saccharomyces cerevisiae)
E318A
mutation has no effect on activity
E356A
mutation has no effect on activity
E405/H406A
double mutant no longer supports growth, the loss of activity is not caused by a failure of Alg11 expression
E405A
E405A/E413
double mutant no longer supports growth, the loss of activity is not caused by a failure of Alg11 expression
E405A/E413A
completely inactive mutant enzyme
E413A
F407A
mutation is not detrimental for growth, impairment of glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G84A
mutation has no effect on growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G84P
mutation reduces growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G85A
mutation has no effect on growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G85P
mutation reduces growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G87A
mutation has no effect on growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
G87P
mutation reduces growth and glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
H406A
mutation is not detrimental for growth, impairment of glycosylation of the vacuolar model glycoproteincarboxypeptidase Y
K302A
mutation has no effect on activity
K319A
mutation causes loss of Alg11 activity
K322A
mutation has no effect on activity
K343A
mutation has no effect on activity
V412A
mutation is not detrimental for growth
additional information