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2.4.1.129: peptidoglycan glycosyltransferase

This is an abbreviated version!
For detailed information about peptidoglycan glycosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.129

Reaction

[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol
+
GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
=
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol
 +
undecaprenyl diphosphate

Synonyms

bacterial cell wall glycosyltransferase, bactoprenyldiphospho-N-acetylmuramoyl-(N-acetyl-D-glucosaminyl)-pentapeptide:peptidoglycan N-acetylmuramoyl-N-acetyl-D-glucosaminyltransferase, bifunctional penicillin-binding protein, class A penicillin-binding protein, class B penicillin-binding protein, DD-transpeptidase, ftsI, glycosyltransferase, peptidoglycan, glycosyltransferase/acyltransferase penicillin-binding protein 4, GTase, MGT, monofunctional glycosyltransferase, mrcB, MtgA, murein synthase, PBP, PBP 1a, PBP 2b, PBP 2x, PBP 3, PBP-1A, PBP-1B, PBP-1C, PBP-2, PBP1, PBP1a, PBP1b, PBP1c, PBP2, PBP2A, PBP2b, PBP2c, PBP2d, PBP3, PBP4, PBP7, PBP8, PBPA, PenA, penicillin binding protein, penicillin binding protein 1b, penicillin-binding protein, penicillin-binding protein 1a, penicillin-binding protein 1B, penicillin-binding protein 2, penicillin-binding protein 3, peptidoglycan glycosyltransferase, peptidoglycan transglycosylase, PG-II, PGT, SgtB, SpoIID

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.129 peptidoglycan glycosyltransferase

Purification

Purification on EC 2.4.1.129 - peptidoglycan glycosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 isozymes
-
expression of the soluble form of the glycosyltransferase domain of PBP1a in Escherichia coli
gene mrcB, recombinant His-tagged PBP1B from Escherichia coli strain BL21 pDML924 membrane fraction by ultracentrifugation, nickel affinity chromatography, dialysis, and tag cleavage by thrombin, followed by dialysis, cation exchange chromatography and again diaylsis, method overview
Ni2+-affinity column chromatography, gel filtration, and Mono Q ion-exchange chromatography
-
Ni2+-agarose column chromatography
-
Ni2+-NTA agarose column chromatography
Ni2+-Sepharose column chromatography
-
nickel chelation column chromatography and Superdex 200 gel filtration
partial
penicillin-binding protein 3
-
recombinant enzyme
recombinant His-tagged wild-type MtgA and MtgA (D68-R269) mutant from Escherichia coli by nickel affinity chromatography in the presence of 0.7% CHAPS
-
recombinant wild-type and mutant enzymes including gel filtration
-
soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain
-