Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta lambdaDE3 cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 2 M ammonium sulfate, 20% (v/v) ethylene glycol and 0.28 mM lauryldimethylamine oxide | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Moenomycin | - |
Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus NCTC 8325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography, gel filtration, and Mono Q ion-exchange chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Staphylococcus aureus | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Staphylococcus aureus NCTC 8325 | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PBP2 | - |
Staphylococcus aureus |
penicillin-binding protein 2 | bifunctional peptidoglycan glycosyltransferase/transpeptidase | Staphylococcus aureus |