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Literature summary for 2.4.1.129 extracted from
- Kinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus (2006), J. Bacteriol., 188, 2528-2532.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E100Q | mutant of the soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT. glycosyltransferase activity of the mutant is reduced 500fold compared to the wild type | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | the enzyme is almost inactive in presence of EDTA | Staphylococcus aureus |  |
| Moenomycin | 75 nM, 50% inhibition | Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mn2+ | Staphylococcus aureus | |
| 0.0028 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 6.0, 10 mM Mn2+ | Staphylococcus aureus | |
| 0.0076 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 50 mM Mg2+ | Staphylococcus aureus | |
| 0.008 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mg2+ | Staphylococcus aureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, stimulation | Staphylococcus aureus | |
| Mg2+ | 50 mM, required for optimal activity | Staphylococcus aureus | |
| Mn2+ | 10 mM required for optimal activity | Staphylococcus aureus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc + undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | - |
Staphylococcus aureus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0063 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 6.0, 10 mM Mn2+ | Staphylococcus aureus | |
| 0.0076 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 50 mM Mg2+ | Staphylococcus aureus | |
| 0.008 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mg2+ | Staphylococcus aureus | |
| 0.013 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mn2+ | Staphylococcus aureus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Staphylococcus aureus |
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