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2.3.1.157: glucosamine-1-phosphate N-acetyltransferase

This is an abbreviated version!
For detailed information about glucosamine-1-phosphate N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.157

Reaction

acetyl-CoA
+
alpha-D-glucosamine 1-phosphate
=
CoA
 +
N-acetyl-alpha-D-glucosamine 1-phosphate

Synonyms

amino-sugar-1-P AcTase, amino-sugar-1-phosphate acetyltransferase, bifunctional GlmU protein, bifunctional N-acetyltransferase/uridylyltransferase, bifunctional protein GlmU, bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase, galactosamine-1-phosphate acetyltransferase, GalN-1-P AcTase, GlcN-1-P acetyltransferase, GlcN-1-P AcTase, GlcNAc-1-P uridyltransferase, GlmU, GlmU acetyltransferase, GlmU enzyme, GlmU uridyltransferase, glucosamine 1-phosphate N-acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase, glucosamine-1-phosphate acetyltransferase, glucosamine-1-phosphate acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase, More, MtbGlmU, MtGlmU, N-acetylglucosamine-1-phosphate pyrophosphorylase, N-acetylglucosamine-1-phosphate uridyltransferase, N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase, N-acetylglucosamine-1-phosphate-uridylyltransferase/glucosamine-1-phosphate-acetyltransferase, Rv1018c, Rxn-1, ST0452, ST0452 protein, STK_04520, UDP-GlcNAc pyrophosphorylase, UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.157 glucosamine-1-phosphate N-acetyltransferase

Cloned

Cloned on EC 2.3.1.157 - glucosamine-1-phosphate N-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli as a GST-fusion protein
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
expression in Escherichia coli
expression in Escherichia coli, expression in Mycobacterium smegmatis ZW-2, a GlmU knoukout strain, activity of GlmU is required for growth of Mycobacterium smegmatis, a gradually reduced enzyme activity in a temperature shift experiment shows that the Mycobacterium smegmatis cells became non-viable and their morphology changes from a normal rod shape to stubby-rounded morphology
expression of mutant enzymes in Escherichia coli
gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene glmU, expression in Escherichia coli strain BL21(DE3)
gene glmU, expression in Escherichia coli strain HMS174(DE3)
gene glmU, expression of GlmU in Lactobacillus casei strain BL309 or PL32, and functional co-overexpression in Lactobacillus casei strain BL23 with genes glmS and glmM encoding GlmS, glucosamine-6-phosphate synthase, and GlmM, phosphoglucosamine mutase, to reconstruct biosynthesis of UDP-N-acetylglucosamine, subcloning in Escherichia coli strain M15 and Lactobacillus lactis strain MG1363
-
gene glmU, expression of N-terminally His-tagged wild-type GlmU and mutant GlmUs in Escherichia coli strain BL21(DE3)
-
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3)
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glmU, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glmU, recombinant expression of N-terminally FLAG-tagged enzyme
gene ST0452, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
GlmU gene cloned from genomic DNA by PCR and inserted into pET3a
-