Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | presence required | Yersinia pestis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Yersinia pestis |
gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Yersinia pseudotuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C307S | site-directed mutagenesis | Yersinia pseudotuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-nitro-5-thiocyanatobenzoic acid | 0.5 mM, 5% residual activity | Yersinia pestis | |
5,5'-dithiobis-(2-nitrobenzoic acid) | 0.1 mM, 33% residual activity | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C | Yersinia pestis | |
0.3 | - |
alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis | |
0.52 | - |
acetyl-CoA | pH 8.2, 37°C | Yersinia pestis | |
0.52 | - |
acetyl-CoA | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Yersinia pseudotuberculosis | |
Mg2+ | required for both UDP-N-acetylglucosamine pyrophosphorylase and glucosamine-1-phosphate N-acetyltransferase activities, optimum concentration 5 mM | Yersinia pestis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52965 | - |
MALDI-TOF MS, SDS-PAGE | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Yersinia pseudotuberculosis | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Yersinia pseudotuberculosis YPIII | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | - |
- |
- |
Yersinia pestis YPIII | - |
- |
- |
Yersinia pseudotuberculosis | - |
- |
- |
Yersinia pseudotuberculosis YPIII | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Yersinia pseudotuberculosis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Yersinia pestis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Yersinia pseudotuberculosis YPIII | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Yersinia pestis YPIII | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52965, recombinant His6-tagged enzyme, SDS-PAGE and mass spectrometry | Yersinia pseudotuberculosis |
More | C324 does not form a disulphide bond with any other cysteine. This is consistent with the replacement of C307 by a serine in Y. pestis and the great distance between the three other cysteines (C296, C301 and C385) and C324 | Yersinia pseudotuberculosis |
Synonyms | Comment | Organism |
---|---|---|
bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase | - |
Yersinia pestis |
GlcN-1-P acetyltransferase | - |
Yersinia pseudotuberculosis |
GlmU | - |
Yersinia pseudotuberculosis |
GlmU | - |
Yersinia pestis |
UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Yersinia pseudotuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
828 | - |
alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C | Yersinia pestis | |
828 | - |
alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis | |
853 | - |
acetyl-CoA | pH 8.2, 37°C | Yersinia pestis | |
853 | - |
acetyl-CoA | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Yersinia pseudotuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Yersinia pseudotuberculosis |
General Information | Comment | Organism |
---|---|---|
evolution | the GlmU proteins encoded by Yersinia pestis and Yersinia pseudotuberculosis are identical in amino acid sequence | Yersinia pseudotuberculosis |
physiological function | the bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase enzyme GlmU is an essential gene in Yersinia | Yersinia pseudotuberculosis |