2.1.1.309: 18S rRNA (guanine1575-N7)-methyltransferase
This is an abbreviated version!
For detailed information about 18S rRNA (guanine1575-N7)-methyltransferase, go to the full flat file.
Reaction
Synonyms
18S rRNA methyltransferase, BUD23, rRNA methyltransferase Bud23, WBSCR22, WBSCR22-TRMT112
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.309 - 18S rRNA (guanine1575-N7)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + guanine10 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
S-adenosyl-L-methionine + guanine1575 in 18S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
m7G1639 methylation
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S-adenosyl-L-methionine + guanine10 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
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conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome, Bud23 methylates G1575 in 18S rRNA in a helix that stacks coaxially with the central pseudoknot. Bud23 protein, but not its methyltransferase activity, is important for its function
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S-adenosyl-L-methionine + guanine10 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
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Site A2 is the primary cleavage site for separating the precursors of 18S and 25S rRNAs
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S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
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S-adenosyl-L-methionine + guanine1575 in 18S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
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S-adenosyl-L-methionine + guanine1575 in 18S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
Bud23 is active in 18S rRNA N7G methylation as a heterodimer in a complex with Trm112. Trm112 is a small zinc finger protein that interacts with and activates three class I MTases in addition to Bud23. N7-methylguanosine introduced at position 1575 on 18S rRNA by Bud23-Trm112 is at a ridge forming a steric block between P- and E-site tRNAs. G1575 is coordinated by a network of conserved Bud23 residues, overview
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secondary structure of the 18S rRNA, overview
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additional information
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secondary structure of the 18S rRNA, overview
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additional information
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Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo
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BUD23 physically interacts with middle-stage 40S biogenesis factors
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Bud23-Trm112 interacts directly with the box C/D snoRNA U3-associated DEAH RNA helicase Dhr1 supposedly involved in central pseudoknot formation. Bud23Trm112 might also contribute to controlling formation of this irreversible and dramatic structural reorganization essential to overall folding of small subunit rRNA
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