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Literature summary for 2.1.1.309 extracted from

  • Sardana, R.; White, J.P.; Johnson, A.W.
    The rRNA methyltransferase Bud23 shows functional interaction with components of the SSU processome and RNase MRP (2013), RNA, 19, 828-840.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information generation of a DELTAbud23 deletion mutant strain which is mated with a subset of temperature-sensitive mutant strains, sporulated, and dissected at room temperature, growth and fitness analysis of the cells. The RNase MRP components Pop3, Pop4, and Snm1 show strong negative genetic interaction with DELTAbud23. Deletion of BUD23 results in steady-state enrichment of nucleoplasmic localization of several small subunit processome components. Utp14 complexes in the absence of Bud23 lack several ribosomal proteins, possibly reflecting stalled assembly intermediates. The lack of disassembly may also be indirectly responsible for the nuclear export defect in the DELTAbud23 mutant Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
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Saccharomyces cerevisiae 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae BUD23 physically interacts with middle-stage 40S biogenesis factors ?
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?
S-adenosyl-L-methionine + guanine10 in tRNA Saccharomyces cerevisiae conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome, Bud23 methylates G1575 in 18S rRNA in a helix that stacks coaxially with the central pseudoknot. Bud23 protein, but not its methyltransferase activity, is important for its function S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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diverse strains
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information BUD23 physically interacts with middle-stage 40S biogenesis factors Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + guanine10 in tRNA conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome, Bud23 methylates G1575 in 18S rRNA in a helix that stacks coaxially with the central pseudoknot. Bud23 protein, but not its methyltransferase activity, is important for its function Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
?
S-adenosyl-L-methionine + guanine10 in tRNA Site A2 is the primary cleavage site for separating the precursors of 18S and 25S rRNAs Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
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?

Synonyms

Synonyms Comment Organism
BUD23
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Saccharomyces cerevisiae
rRNA methyltransferase Bud23
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction DELTAbud23 mutants have severely reduced small subunit levels and showa general failure in cleavage at site A2 during rRNA processing. A2 cleavage in a DELTAbud23 mutant is inefficient, with a marked reduction in the levels of 27S A2 intermediate. In the absence of A2 cleavage, cleavage at A3 is likely to be essential for separating the precursors for 40S and 60S processing. The strong negative genetic interaction observed between DELTAbud23 and RNase MRP components may reflect such a block in rRNA processing. Utp14 particle in a DELTAbud23 mutant contains U3 snoRNA but lacks 20S pre-rRNA. Phenotypes, overview. Although BUD23 is not essential for viability, deletion of the gene results in a severe slow-growth phenotype Saccharomyces cerevisiae
physiological function Bud23 is responsible for the conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome. Bud23 protein, but not its methyltransferase activity, is important for its function. Bud23 is required for efficient A2 cleavage. Bud23 is required for the proper localization of small subunit components, UTP proteins mislocalize to the nucleoplasm in the absence of Bud23 Saccharomyces cerevisiae