2.1.1.309: 18S rRNA (guanine1575-N7)-methyltransferase
This is an abbreviated version!
For detailed information about 18S rRNA (guanine1575-N7)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.309
Reaction
Synonyms
18S rRNA methyltransferase, BUD23, rRNA methyltransferase Bud23, WBSCR22, WBSCR22-TRMT112
ECTree
2.1.1.309 18S rRNA (guanine1575-N7)-methyltransferaseAdvanced search results
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results (Engineering
Engineering on EC 2.1.1.309 - 18S rRNA (guanine1575-N7)-methyltransferase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D77A
site-directed mutagenesis, methylation inactive mutant, 25% remaining S-adenosyl-L-methionine binding activity
I31W
site-directed mutagenesis, methylation inactive mutant, 25% remaining S-adenosyl-L-methionine binding activity
K21E/R27E
site-directed mutagenesis, methylation inactive mutant
S118E
site-directed mutagenesis, methylation and S-adenosyl-L-methionine binding inactive mutant
S118R
site-directed mutagenesis, methylation and S-adenosyl-L-methionine binding inactive mutant
Y159A
site-directed mutagenesis, methylation inactive mutant, 50% remaining S-adenosyl-L-methionine binding activity
additional information
additional information
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generation of a DELTAbud23 deletion mutant strain which is mated with a subset of temperature-sensitive mutant strains, sporulated, and dissected at room temperature, growth and fitness analysis of the cells. The RNase MRP components Pop3, Pop4, and Snm1 show strong negative genetic interaction with DELTAbud23. Deletion of BUD23 results in steady-state enrichment of nucleoplasmic localization of several small subunit processome components. Utp14 complexes in the absence of Bud23 lack several ribosomal proteins, possibly reflecting stalled assembly intermediates. The lack of disassembly may also be indirectly responsible for the nuclear export defect in the DELTAbud23 mutant
additional information
protein levels and cell growth of mutants compared to wild-type, overview. Reconstitution of Dhr1-Bud23-Trm112 ternary complex by mixing Bud23-Trm112 with a 1.5 M excess of Dhr1[58-270] in 20 mM Tris·HCl, pH 7.5, 50 mM NaCl, 0.010 mM ZnCl2, 5 mM 2-mercaptoethanol
