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2.1.1.230: 23S rRNA (adenosine1067-2'-O)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (adenosine1067-2'-O)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.230

Reaction

S-adenosyl-L-methionine
+
adenosine1067 in 23S rRNA
=
S-adenosyl-L-homocysteine
 +
2'-O-methyladenosine1067 in 23S rRNA

Synonyms

EC 2.1.1.66, NHR, NHR protein, nosiheptide resistance methyltransferase, nosiheptide-resistance methyltransferase, thiostrepton resistance methyltransferase, thiostrepton-resistance methylase, thiostrepton-resistance methyltransferase, TSR

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.230 23S rRNA (adenosine1067-2'-O)-methyltransferase

Engineering

Engineering on EC 2.1.1.230 - 23S rRNA (adenosine1067-2'-O)-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D36A
mutation significantly decreases activity
E220Q
mutant has 10% of activity compared to wild-type enzyme
E35A
mutation significantly decreases activity
E91A
mutation significantly decreases activity
F88A
mutation significantly decreases activity
H258A/E259A
double mutaion decreases methyltransferase activity to 40% compared to wild-type activity
R135A
R165A
mutation significantly decreases activity
R92A
mutation significantly decreases activity
S219A
mutation slightly decreases activity
R162A
site-directed mutagenesis, the mutant binds RNA with 100fold weaker affinity than wild-type Tsr
R26A
site-directed mutagenesis, the mutant is unable to promote the RNase V1-sensitive RNA structural changes, but maintains its interaction with the RNA under certain conditions for the protection of nucleotides G1068 and G1071 from cleavage by RNase T1