Crystallization (Comment) | Organism |
---|---|
crystal structures of the enzyme and the enzyme in complex with S-adenosyl-L-methionine at 2.0 and 2.1 A resolution, respectively | Streptomyces actuosus |
Protein Variants | Comment | Organism |
---|---|---|
D36A | mutation significantly decreases activity | Streptomyces actuosus |
E220Q | mutant has 10% of activity compared to wild-type enzyme | Streptomyces actuosus |
E35A | mutation significantly decreases activity | Streptomyces actuosus |
E91A | mutation significantly decreases activity | Streptomyces actuosus |
F88A | mutation significantly decreases activity | Streptomyces actuosus |
H258A/E259A | double mutaion decreases methyltransferase activity to 40% compared to wild-type activity | Streptomyces actuosus |
R135A | mutation significantly decreases activity | Streptomyces actuosus |
R165A | mutation significantly decreases activity | Streptomyces actuosus |
R92A | mutation significantly decreases activity | Streptomyces actuosus |
S219A | mutation slightly decreases activity | Streptomyces actuosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces actuosus | P52391 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces actuosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenosine1067 in Escherichia coli 23S rRNA | - |
Streptomyces actuosus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in Escherichia coli 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | dimerization is required for methyltransferase activity | Streptomyces actuosus |
Synonyms | Comment | Organism |
---|---|---|
nosiheptide-resistance methyltransferase | - |
Streptomyces actuosus |
General Information | Comment | Organism |
---|---|---|
physiological function | methylation at adenosine1067 in 23S rRNA is essential for resistance against nosiheptide, a sulfur peptide antibiotic, which is produced by the nosiheptide-producing strain, Streptomyces actuosus | Streptomyces actuosus |