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Literature summary for 2.1.1.230 extracted from

  • Yang, H.; Wang, Z.; Shen, Y.; Wang, P.; Jia, X.; Zhao, L.; Zhou, P.; Gong, R.; Li, Z.; Yang, Y.; Chen, D.; Murchie, A.I.; Xu, Y.
    Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus (2010), Biochemistry, 49, 6440-6450.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of the enzyme and the enzyme in complex with S-adenosyl-L-methionine at 2.0 and 2.1 A resolution, respectively Streptomyces actuosus

Protein Variants

Protein Variants Comment Organism
D36A mutation significantly decreases activity Streptomyces actuosus
E220Q mutant has 10% of activity compared to wild-type enzyme Streptomyces actuosus
E35A mutation significantly decreases activity Streptomyces actuosus
E91A mutation significantly decreases activity Streptomyces actuosus
F88A mutation significantly decreases activity Streptomyces actuosus
H258A/E259A double mutaion decreases methyltransferase activity to 40% compared to wild-type activity Streptomyces actuosus
R135A mutation significantly decreases activity Streptomyces actuosus
R165A mutation significantly decreases activity Streptomyces actuosus
R92A mutation significantly decreases activity Streptomyces actuosus
S219A mutation slightly decreases activity Streptomyces actuosus

Organism

Organism UniProt Comment Textmining
Streptomyces actuosus P52391
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces actuosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenosine1067 in Escherichia coli 23S rRNA
-
Streptomyces actuosus S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in Escherichia coli 23S rRNA
-
?

Subunits

Subunits Comment Organism
homodimer dimerization is required for methyltransferase activity Streptomyces actuosus

Synonyms

Synonyms Comment Organism
nosiheptide-resistance methyltransferase
-
Streptomyces actuosus

General Information

General Information Comment Organism
physiological function methylation at adenosine1067 in 23S rRNA is essential for resistance against nosiheptide, a sulfur peptide antibiotic, which is produced by the nosiheptide-producing strain, Streptomyces actuosus Streptomyces actuosus