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2.1.1.190: 23S rRNA (uracil1939-C5)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (uracil1939-C5)-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
uracil1939 in 23S rRNA
=
S-adenosyl-L-homocysteine
 +
5-methyluracil1939 in 23S rRNA

Synonyms

m5U methyltransferase, Mcap0476, RlmCD, RlmD, RlmFO, RNA m5U methyltransferase, RNA uridine methyltransferase A, RumA, SP 1029, SP_1029, YefA, YgcA

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.190 23S rRNA (uracil1939-C5)-methyltransferase

Engineering

Engineering on EC 2.1.1.190 - 23S rRNA (uracil1939-C5)-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D265A
the specific activity of the mutant enzyme is 830fold lower than that of the wild-type enzyme
D265E
mutant enzyme has no activity
D363A
mutation results in complete loss of activity
D363N
the specific activity of the mutant enzyme is 2fold lower than that of the wild-type enzyme
E424Q
the mutant enzyme displays a biphasic reaction profile with an initial burst phase followed by a slow second phase, mutation results in at least 350fold reduction in the rate of proton abstraction
D381A
2% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 40% residual activity towards U1939 RNA substrate
F145A
5% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 120% activity towards U1939 RNA substrate
H151A
5% residual activity towards U747 RNA substrate,reaction of EC 2.11.189, 55% activity towards U1939 RNA substrate
N249A
35% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 55% residual activity towards U1939 RNA substrate
Q162A
18% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 160% activity towards U1939 RNA substrate
Q283A
8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 50% residual activity towards U1939 RNA substrate
R127A
8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 2% residual activity towards U1939 RNA substrate