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Literature summary for 2.1.1.190 extracted from

  • Jiang, Y.; Yu, H.; Li, F.; Cheng, L.; Zhu, L.; Shi, Y.; Gong, Q.
    Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD (2018), PLoS Pathog., 14, e1007379 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.190

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of RlmCD in complex with its cofactor and the RNA substrate containing U747 at 2.00 A or U1939 at 3.10 A. The side-chain rearrangement of F145 displays an unusual mechanism through which RlmCD can discriminate between U747- and U1939-containing RNA substrate by switching the intermolecular aromatic stacking between protein and RNA on/off Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
D381A 2% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 40% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
F145A 5% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 120% activity towards U1939 RNA substrate Streptococcus pneumoniae
H151A 5% residual activity towards U747 RNA substrate,reaction of EC 2.11.189, 55% activity towards U1939 RNA substrate Streptococcus pneumoniae
N249A 35% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 55% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
Q162A 18% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 160% activity towards U1939 RNA substrate Streptococcus pneumoniae
Q283A 8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 50% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
R127A 8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 2% residual activity towards U1939 RNA substrate Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ?
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additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ATCC BAA-334 ?
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Synonyms

Synonyms Comment Organism
RlmCD
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 Streptococcus pneumoniae
SP_1029
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 Streptococcus pneumoniae